Bone marrow-derived hematopoietic cells undergo myogenic differentiation following a pax-7 independent pathway

Alexandros Xynos, Paola Corbella, Nathalie Belmonte, Roberta Zini, Rossella Manfredini, Giuliana Ferrari

Research output: Contribution to journalArticlepeer-review

Abstract

Several reports showed that hematopoietic stem cells (HSCs) participate in muscle regeneration, raising hope for their therapeutic potential for degenerative muscle diseases. However, proof that HSCs are able to reprogram their fate and enter a myogenic pathway, remains elusive. We demonstrate that murine bone marrow (BM)-derived hematopoietic cells, carrying reporter genes controlled by muscle-specific regulatory elements from the Myf5, myosin light chain (MLC3F), or MCK genes, are induced by myoblasts to activate muscle-specific genes. This potential resides in the more undifferentiated progenitors, expressing surface markers typical of HSCs. Comparative gene expression profiling of CD45+/Sca1+ cells isolated from muscle or BM shows that hematopoietic cells participate to muscle regeneration, by undergoing a profound although incomplete myogenic reprogramming on interaction with the muscle microenviroment. These cells undergo specification and differentiation independently from Pax7 and MyoD, and lack Pax7-associated properties, such as self-renewal and proliferation, distinguishing from satellite cells. Our findings indicate that hematopoietic cells, on seeding in the muscle, become a distinct cell population endowed with myogenic potential.

Original languageEnglish
Pages (from-to)965-973
Number of pages9
JournalStem Cells
Volume28
Issue number6
Publication statusPublished - Jun 2010

Keywords

  • Bone marrow
  • Hematopoietic stem cells
  • Myogenesis
  • Skeletal muscle

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine

Fingerprint Dive into the research topics of 'Bone marrow-derived hematopoietic cells undergo myogenic differentiation following a pax-7 independent pathway'. Together they form a unique fingerprint.

Cite this