Bone marrow mitogen-stimulated direct antiglobulin test in a case of erythroblastic synartesis

Anna Zaninoni, Francesca G. Imperiali, Mauro Pomati, Mariangela Colombi, Carla Boschetti, Wilma Barcellini

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In this article we report a case of erythroblastic synartesis, a rare disease characterized by ineffective erythropoiesis, clusters of erythroblasts due to membrane invaginations, in which an autoimmune pathogenesis is hypothesized. We investigated the presence of anti-erythroblast autoimmunity in bone marrow cultures using a mitogen-stimulated direct antiglobulin test, a method reported to be able to disclose a latent autoimmunity in various diseases. The test revealed the presence of erythroblast-bound IgG, supporting the hypothesis of the autoimmune pathogenesis of erythroblastic synartesis. Supernatants induced the same specific morphological features, i.e erythroblastic clustering and diserythropoietic signs (multiple nuclei, nuclear inclusions, and intercellular bridges) in normal progenitors.

Original languageEnglish
Pages (from-to)459-462
Number of pages4
JournalClinical Laboratory
Volume56
Issue number9-10
Publication statusPublished - 2010

Fingerprint

Coombs Test
Erythroblasts
Mitogens
Anti-Idiotypic Antibodies
Bone
Bone Marrow
Autoimmunity
Intranuclear Inclusion Bodies
Immunoglobulin G
Erythropoiesis
Rare Diseases
Membranes
Cluster Analysis

Keywords

  • Anti-erythroblast autoimmunity
  • Bone marrow
  • Erythroblastic synartesis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Bone marrow mitogen-stimulated direct antiglobulin test in a case of erythroblastic synartesis. / Zaninoni, Anna; Imperiali, Francesca G.; Pomati, Mauro; Colombi, Mariangela; Boschetti, Carla; Barcellini, Wilma.

In: Clinical Laboratory, Vol. 56, No. 9-10, 2010, p. 459-462.

Research output: Contribution to journalArticle

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