Bone marrow molecular analysis of yt-cell receptor gene rearrangement in a child with acute lymphoblastic leukemia is not predictive for cns relapse

G. Anselml, D. Di Martino, F. Scuderi, V. Chiesa, C. Micalizzi, M. Lanciottl, A. Valetta, G. Dini, P. Comao, Ua Ferraris, P. G. Mori

Research output: Contribution to journalArticlepeer-review

Abstract

We have studied the Minimal Residual Disease (MRD) in a 6 years old patient with T-Acute Lymphoblastic Leukemia (T-ALL). His genomic DNA, extracted from bone marrow (BM), was evaluated for the Vy1-Jr1323 rearrangement of yT-cell receptor gene (TCRG) using polymerase chain reaction (PCR) amplification. The amplified DNA fragment was sequenced and a Patient's Specific Primer (PSP) was synthetized: Vγ2 Jγ12 ........TGTGCC...................GTTCACTAAAG................AATTAT. We used the slot blot technique to carry out a molecular followup (described below) by hybridizing BM-cells DNA with PSP. The leukemic clone reappared 3 mounths before any morphological change. This event explains the first BM relapse, but not the relapse observed in the central nervous system (CNS) and in lynphonodes (LN) after allogeneic BM transplant. Intact, the child relapsed in both regional LN and CNS being in hematological and molecular remission Moleculler follow-up (days) [LtflwdL HR. Hwntfotoglc! Mid ctMcal mtitMfcn; 1R, flrat BM ratapM; 2R. Mcomt BM raiipM, BMT, oonungulnMNM HLA oontpvllbt Hogm: bon marrow Iraniptant. LN, lymptinodal ratapM; CNS central nwvou tyrtwn ratapM] LN HR HR HR HR 1R BMT HR 2R 2 BMT CNS + +- - - - - - - + + - - - + + - Ml 14 42 N 120 210 170 MO 4 HO TM II 0 ITO K 10M 1110 1170 1200 1210 1310 Our future aim is to study DNA from cerebrospinal fluid and paraffin embedded section of salivary glands and LN and to verify if these districts show the same sequence probed by PSP and so the original leukemic clone.

Original languageEnglish
Pages (from-to)797
Number of pages1
JournalExperimental Hematology
Volume26
Issue number8
Publication statusPublished - 1998

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

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