Bone marrow stromal cells and their use in regenerating bone tissue

Rodolfo Quarto, Giordano Bianchi, Milena Mastrogiacomo, Anita Muraglia, Ranieri Cancedda

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This work developed a new technique for in situ synthesis of oligonucleotide arrays on glass surfaces. In this method, based on the standard phosphoramidites chemistry protocol, the coupling was conducted through contacting glass surfaces with a set of molecular stamps on which surfaces spread nucleoside monomer and tetrazole mixed acetonitrile solution. It was shown that 20-mer oligonucleotide on the glass slide was successfully synthesized using the modified polydimethylsiloxane (PDMS) stamps and showed the high synthetic efficiency. An effective method has been used to eliminate residual reactive nucleosides on chip with small molecules containing hydroxyl group. A specific oligonucleotide arrays of four probes including matched and mismatched with the target sequence was fabricated to identify the perfect match and mismatch sequences. It indicated that the perfect match and mismatch probes hybridization fluorescent signals had clearly difference, and may be used to identify rapidly screen single-nucleotide polymorphisms.

Original languageEnglish
Title of host publicationProceedings of the IEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages33
Number of pages1
ISBN (Print)0780375572, 9780780375574
DOIs
Publication statusPublished - 2002
EventIEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002 - Genoa, Italy
Duration: Jun 6 2002Jun 9 2002

Other

OtherIEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002
CountryItaly
CityGenoa
Period6/6/026/9/02

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ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering
  • Biotechnology

Cite this

Quarto, R., Bianchi, G., Mastrogiacomo, M., Muraglia, A., & Cancedda, R. (2002). Bone marrow stromal cells and their use in regenerating bone tissue. In Proceedings of the IEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002 (pp. 33). [1174990] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MCTE.2002.1174990