Bone turnover in wild type and pleiotrophin-transgenic mice housed for three months in the international space station (ISS)

Sara Tavella, Alessandra Ruggiu, Alessandra Giuliani, Francesco Brun, Barbara Canciani, Adrian Manescu, Katia Marozzi, Michele Cilli, Delfina Costa, Yi Liu, Federica Piccardi, Roberta Tasso, Giuliana Tromba, Franco Rustichelli, Ranieri Cancedda

Research output: Contribution to journalArticle

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Abstract

Bone is a complex dynamic tissue undergoing a continuous remodeling process. Gravity is a physical force playing a role in the remodeling and contributing to the maintenance of bone integrity. This article reports an investigation on the alterations of the bone microarchitecture that occurred in wild type (Wt) and pleiotrophin-transgenic (PTN-Tg) mice exposed to a near-zero gravity on the International Space Station (ISS) during the Mice Drawer System (MDS) mission, to date, the longest mice permanence (91 days) in space. The transgenic mouse strain over-expressing pleiotrophin (PTN) in bone was selected because of the PTN positive effects on bone turnover. Wt and PTN-Tg control animals were maintained on Earth either in a MDS payload or in a standard vivarium cage. This study revealed a bone loss during spaceflight in the weight-bearing bones of both strains. For both Tg and Wt a decrease of the trabecular number as well as an increase of the mean trabecular separation was observed after flight, whereas trabecular thickness did not show any significant change. Non weight-bearing bones were not affected. The PTN-Tg mice exposed to normal gravity presented a poorer trabecular organization than Wt mice, but interestingly, the expression of the PTN transgene during the flight resulted in some protection against microgravity's negative effects. Moreover, osteocytes of the Wt mice, but not of Tg mice, acquired a round shape, thus showing for the first time osteocyte space-related morphological alterations in vivo. The analysis of specific bone formation and resorption marker expression suggested that the microgravity-induced bone loss was due to both an increased bone resorption and a decreased bone deposition. Apparently, the PTN transgene protection was the result of a higher osteoblast activity in the flight mice.

Original languageEnglish
Article numbere33179
JournalPLoS One
Volume7
Issue number3
DOIs
Publication statusPublished - Mar 15 2012

Fingerprint

Bone Remodeling
Space stations
Transgenic Mice
Bone
bones
genetically modified organisms
Bone and Bones
mice
Weightlessness
Osteocytes
Bearings (structural)
microgravity
Weight-Bearing
Gravitation
bone resorption
flight
Bone Resorption
Transgenes
gravity
Microgravity

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Tavella, S., Ruggiu, A., Giuliani, A., Brun, F., Canciani, B., Manescu, A., ... Cancedda, R. (2012). Bone turnover in wild type and pleiotrophin-transgenic mice housed for three months in the international space station (ISS). PLoS One, 7(3), [e33179]. https://doi.org/10.1371/journal.pone.0033179

Bone turnover in wild type and pleiotrophin-transgenic mice housed for three months in the international space station (ISS). / Tavella, Sara; Ruggiu, Alessandra; Giuliani, Alessandra; Brun, Francesco; Canciani, Barbara; Manescu, Adrian; Marozzi, Katia; Cilli, Michele; Costa, Delfina; Liu, Yi; Piccardi, Federica; Tasso, Roberta; Tromba, Giuliana; Rustichelli, Franco; Cancedda, Ranieri.

In: PLoS One, Vol. 7, No. 3, e33179, 15.03.2012.

Research output: Contribution to journalArticle

Tavella, S, Ruggiu, A, Giuliani, A, Brun, F, Canciani, B, Manescu, A, Marozzi, K, Cilli, M, Costa, D, Liu, Y, Piccardi, F, Tasso, R, Tromba, G, Rustichelli, F & Cancedda, R 2012, 'Bone turnover in wild type and pleiotrophin-transgenic mice housed for three months in the international space station (ISS)', PLoS One, vol. 7, no. 3, e33179. https://doi.org/10.1371/journal.pone.0033179
Tavella, Sara ; Ruggiu, Alessandra ; Giuliani, Alessandra ; Brun, Francesco ; Canciani, Barbara ; Manescu, Adrian ; Marozzi, Katia ; Cilli, Michele ; Costa, Delfina ; Liu, Yi ; Piccardi, Federica ; Tasso, Roberta ; Tromba, Giuliana ; Rustichelli, Franco ; Cancedda, Ranieri. / Bone turnover in wild type and pleiotrophin-transgenic mice housed for three months in the international space station (ISS). In: PLoS One. 2012 ; Vol. 7, No. 3.
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