Booster PCR: evaluation of an improved method for amplification of a few HIV-1 proviral DNA sequences.

G. Filice, M. Debiaggi, P. M. Cereda, E. Romero

Research output: Contribution to journalArticle

Abstract

A biphasic polymerase chain reaction designed as booster PCR for the detection of human immunodeficiency virus type-1 (HIV-1) was evaluated in samples containing a very low number of target DNA. We examined DNA samples obtained from chronically infected H9/HTLV-III B cells and purified plasmidic DNA containing the entire HIV-1 genome. By using booster PCR we detected HIV-1 DNA sequences up to 5 infected cells in samples containing about 2 micrograms of genomic DNA, and up to 1 copy of plasmidic DNA in samples containing about 0.5 microgram of genomic DNA. Otherwise by using standard PCR HIV-DNA up 100 infected cells and up to 20 copies from plasmidic DNA could be detected. Our experiments in amplification of HIV-1 proviral DNA have demonstrated that booster PCR enhances sensitivity of detection of standard PCR in small quantities of target sequences at least 20-fold with no loss of specificity.

Original languageEnglish
Pages (from-to)129-133
Number of pages5
JournalNew Microbiologica
Volume16
Issue number2
Publication statusPublished - Apr 1993

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Fingerprint Dive into the research topics of 'Booster PCR: evaluation of an improved method for amplification of a few HIV-1 proviral DNA sequences.'. Together they form a unique fingerprint.

  • Cite this