Both CD133+ and CD133- medulloblastoma cell lines express ligands for triggering NK receptors and are susceptible to NK-mediated cytotoxicity

Roberta Castriconi, Alessandra Dondero, Francesca Negri, Francesca Bellora, Paolo Nozza, Barbara Carnemolla, Alessandro Raso, Lorenzo Moretta, Alessandro Moretta, Cristina Bottino

Research output: Contribution to journalArticle

Abstract

Adoptive cellular immunotherapy has been proposed as an additional treatment of medulloblastoma, an intracranial tumor characterized by a particularly poor prognosis. However, little is known on the ability of the immune system to effectively attack this tumor. In this study, we show that activated human NK cells efficiently kill medulloblastoma cell lines in vitro. NK-mediated killing involved different activating receptors (including NKp46, NKp30, DNAM-1 and NKG2D) and correlated with the presence of their specific ligands on tumor cells. In contrast, the absence of major adhesion interactions, such as LFA-1/ICAM did not impair the NK-mediated cytotoxicity. Medulloblastoma expressed a number of tumor-associated molecules including CD146 and CD133, considered a marker for cancer stem cells. Remarkably, both CD133-positive and CD133-negative cell lines were susceptible to lysis. Tumor cells also expressed molecules that are currently used as diagnostic tools for neuroblastoma cell identification. In particular, B7 homolog 3 (B7-H3) was expressed by all the medulloblastoma cell lines analyzed, while the presence of GD2 and NB84 was restricted to given cell lines and/or marked a defined tumor cell subset.

Original languageEnglish
Pages (from-to)3190-3196
Number of pages7
JournalEuropean Journal of Immunology
Volume37
Issue number11
DOIs
Publication statusPublished - Nov 2007

Keywords

  • Activating NK receptors
  • Ligands for activating NK receptors
  • Medulloblastoma
  • Natural killer cells
  • Tumor markers

ASJC Scopus subject areas

  • Immunology

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