Abstract
Colony-forming cells (CFC) and long-term culture-initiating cells (LTC- IC) include a spectrum of progenitor types whose potential contributions to the haemopoietic recovery seen in patients transplanted with mobilized peripheral blood progenitor cells (PBPC) remains unclear. We evaluated both the number and cycling status of the circulating LTC-IC and CFC harvested from 12 patients treated with chemotherapy and G-CSF using a modified 6-week LTC-IC assay. The frequency of the LTC-IC and CFC in the mobilized PB samples were increased 45- and 750-fold, respectively. Interestingly, comparison of these values for PB samples, taken just prior to the start of the leukapheresis, with the progenitor content of the 3 h harvest, showed that, on average, the leukapheresis product contained 19 times more LTC-IC (P3H-Tdr = 70 ± 8%, n = 9). On the other hand, the majority of the CFC in the apheresis collections were initially quiescent (per cent kill after 16 h in 3H-Tdr = 37 ± 6%, n = 12). These findings demonstrate the rapidity with which a primitive subset of LTC-IC may enter the circulation during the early phase of rebound haemopoiesis induced by chemotherapy plus G-CSF and provide evidence of differences in the mechanisms regulating LTC-IC and CFC mobilization.
| Original language | English |
|---|---|
| Pages (from-to) | 394-402 |
| Number of pages | 9 |
| Journal | British Journal of Haematology |
| Volume | 99 |
| Issue number | 2 |
| Publication status | Published - 1997 |
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Keywords
- Chemotherapy
- Growth factors
- Haemopoiesis
- Leukapheresis
- Stem cell transplantation
ASJC Scopus subject areas
- Hematology
Cite this
Both cycling and noncycling primitive progenitors continue to be mobilized into the circulation during the leukapheresis of patients pretreated with chemotherapy and G-CSF. / Ponchio, Luisa; Eaves, Connie; Hogge, Donna; Perotti, Cesare; Torretta, Lorella; Cerani, Paola; Salvaneschi, Laura; Ascari, Edoardo; Cazzola, Mario.
In: British Journal of Haematology, Vol. 99, No. 2, 1997, p. 394-402.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Both cycling and noncycling primitive progenitors continue to be mobilized into the circulation during the leukapheresis of patients pretreated with chemotherapy and G-CSF
AU - Ponchio, Luisa
AU - Eaves, Connie
AU - Hogge, Donna
AU - Perotti, Cesare
AU - Torretta, Lorella
AU - Cerani, Paola
AU - Salvaneschi, Laura
AU - Ascari, Edoardo
AU - Cazzola, Mario
PY - 1997
Y1 - 1997
N2 - Colony-forming cells (CFC) and long-term culture-initiating cells (LTC- IC) include a spectrum of progenitor types whose potential contributions to the haemopoietic recovery seen in patients transplanted with mobilized peripheral blood progenitor cells (PBPC) remains unclear. We evaluated both the number and cycling status of the circulating LTC-IC and CFC harvested from 12 patients treated with chemotherapy and G-CSF using a modified 6-week LTC-IC assay. The frequency of the LTC-IC and CFC in the mobilized PB samples were increased 45- and 750-fold, respectively. Interestingly, comparison of these values for PB samples, taken just prior to the start of the leukapheresis, with the progenitor content of the 3 h harvest, showed that, on average, the leukapheresis product contained 19 times more LTC-IC (P3H-Tdr = 70 ± 8%, n = 9). On the other hand, the majority of the CFC in the apheresis collections were initially quiescent (per cent kill after 16 h in 3H-Tdr = 37 ± 6%, n = 12). These findings demonstrate the rapidity with which a primitive subset of LTC-IC may enter the circulation during the early phase of rebound haemopoiesis induced by chemotherapy plus G-CSF and provide evidence of differences in the mechanisms regulating LTC-IC and CFC mobilization.
AB - Colony-forming cells (CFC) and long-term culture-initiating cells (LTC- IC) include a spectrum of progenitor types whose potential contributions to the haemopoietic recovery seen in patients transplanted with mobilized peripheral blood progenitor cells (PBPC) remains unclear. We evaluated both the number and cycling status of the circulating LTC-IC and CFC harvested from 12 patients treated with chemotherapy and G-CSF using a modified 6-week LTC-IC assay. The frequency of the LTC-IC and CFC in the mobilized PB samples were increased 45- and 750-fold, respectively. Interestingly, comparison of these values for PB samples, taken just prior to the start of the leukapheresis, with the progenitor content of the 3 h harvest, showed that, on average, the leukapheresis product contained 19 times more LTC-IC (P3H-Tdr = 70 ± 8%, n = 9). On the other hand, the majority of the CFC in the apheresis collections were initially quiescent (per cent kill after 16 h in 3H-Tdr = 37 ± 6%, n = 12). These findings demonstrate the rapidity with which a primitive subset of LTC-IC may enter the circulation during the early phase of rebound haemopoiesis induced by chemotherapy plus G-CSF and provide evidence of differences in the mechanisms regulating LTC-IC and CFC mobilization.
KW - Chemotherapy
KW - Growth factors
KW - Haemopoiesis
KW - Leukapheresis
KW - Stem cell transplantation
UR - http://www.scopus.com/inward/record.url?scp=0030663385&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030663385&partnerID=8YFLogxK
M3 - Article
C2 - 9375761
AN - SCOPUS:0030663385
VL - 99
SP - 394
EP - 402
JO - British Journal of Haematology
JF - British Journal of Haematology
SN - 0007-1048
IS - 2
ER -