Bound and unbound pyridine dinucleotides in normal and glucose-6-phosphate dehydrogenase-deficient erythrocytes

Letizia Canepa, Anna Maria Ferraris, Maurizio Miglino, Gian Franco Gaetani

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We have measured, by a sensitive cycling assay, the concentration of bound and unbound dinucleotides in normal and glucose-6-phosphate dehydrogenase (G6PD)-deficient erythrocytes. Measurement of free NADP in ultrafiltrates confirms that in normal erythrocytes almost all NADP is bound to cytosolic proteins. In glucose-6-phosphate dehydrogenase-deficient erythrocytes unbound NADP is significantly higher than in normal red cells and the NADP+/NADPH ratio is largely in favor of the oxidized form. In normal and glucose-6-phosphate dehydrogenase-deficient erythrocytes essentially all NAD (bound and unbound) is in the oxidized state. About 50% of the total amount of NAD (NAD+ + NADH) is free in the cytosol, with a NAD+/NADH ratio greater than 100.

Original languageEnglish
Pages (from-to)101-104
Number of pages4
JournalBBA - General Subjects
Volume1074
Issue number1
DOIs
Publication statusPublished - May 24 1991

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Glucosephosphate Dehydrogenase
NAD
NADP
Erythrocytes
Cytosol
pyridine
Assays
Cells
Proteins

Keywords

  • (Human erythrocyte)
  • Glucose-6-phosphate dehydrogenase
  • NAD(H)
  • NADP(H)

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Bound and unbound pyridine dinucleotides in normal and glucose-6-phosphate dehydrogenase-deficient erythrocytes. / Canepa, Letizia; Ferraris, Anna Maria; Miglino, Maurizio; Gaetani, Gian Franco.

In: BBA - General Subjects, Vol. 1074, No. 1, 24.05.1991, p. 101-104.

Research output: Contribution to journalArticle

Canepa, Letizia ; Ferraris, Anna Maria ; Miglino, Maurizio ; Gaetani, Gian Franco. / Bound and unbound pyridine dinucleotides in normal and glucose-6-phosphate dehydrogenase-deficient erythrocytes. In: BBA - General Subjects. 1991 ; Vol. 1074, No. 1. pp. 101-104.
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