Bovine pericardium patch wrapping intestinal anastomosis improves healing process and prevents leakage in a pig model

Mario Testini, Angela Gurrado, Piero Portincasa, Salvatore Scacco, Andrea Marzullo, Giuseppe Piccinni, Germana Lissidini, Luigi Greco, Maria Antonietta De Salvia, Leonilde Bonfrate, Lucantonio Debellis, Nicola Sardaro, Francesco Staffieri, Maria Rosaria Carratù, Antonio Crovace

Research output: Contribution to journalArticle

Abstract

Failure of intestinal anastomosis is a major complication following abdominal surgery. Biological materials have been introduced as reinforcement of abdominal wall hernia in contaminated setting. An innovative application of biological patch is its use as reinforcement of gastrointestinal anastomosis. The aim of study was to verify whether the bovine pericardium patch improves the healing of anastomosis, when in vivo wrapping the suture line of pig intestinal anastomosis, avoiding leakage in the event of deliberately incomplete suture. Forty-three pigs were randomly divided: Group 1 (control, n = 14): hand-sewn ileo-ileal and colo-colic anastomosis; Group 2 (n = 14): standard anastomosis wrapped by pericardium bovine patch; Group 3 (n = 1) and 4 (n = 14): one suture was deliberately incomplete and also wrapped by patch in the last one. Intraoperative evaluation, histological, biochemical, tensiometric and electrophysiological studies of intestinal specimens were performed at 48 h, 7 and 90 days after. In groups 2 and 4, no leak, stenosis, abscess, peritonitis, mesh displacement or shrinkage were found and adhesion rate decreased compared to control. Biochemical studies showed mitochondrial function improvement in colic wrapped anastomosis. Tensiometric evaluations suggested that the patch preserves the colic contractility similar to the controls. Electrophysiological results demonstrated that the patch also improves the mucosal function restoring almost normal transport properties. Use of pericardium bovine patch as reinforcement of intestinal anastomosis is safe and effective, significantly improving the healing process. Data of prevention of acute peritonitis and leakage in cases of iatrogenic perforation of anastomoses, covered with patch, is unpublished.

Original languageEnglish
Article numbere86627
JournalPLoS One
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 29 2014

Fingerprint

pericardium
Colic
Pericardium
colic
sutures
Sutures
Reinforcement
Swine
peritonitis
Peritonitis
swine
cattle
Abdominal Hernia
hernia
Abdominal Wall
abscess
shrinkage
Biological materials
Transport properties
Abscess

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Testini, M., Gurrado, A., Portincasa, P., Scacco, S., Marzullo, A., Piccinni, G., ... Crovace, A. (2014). Bovine pericardium patch wrapping intestinal anastomosis improves healing process and prevents leakage in a pig model. PLoS One, 9(1), [e86627]. https://doi.org/10.1371/journal.pone.0086627

Bovine pericardium patch wrapping intestinal anastomosis improves healing process and prevents leakage in a pig model. / Testini, Mario; Gurrado, Angela; Portincasa, Piero; Scacco, Salvatore; Marzullo, Andrea; Piccinni, Giuseppe; Lissidini, Germana; Greco, Luigi; De Salvia, Maria Antonietta; Bonfrate, Leonilde; Debellis, Lucantonio; Sardaro, Nicola; Staffieri, Francesco; Carratù, Maria Rosaria; Crovace, Antonio.

In: PLoS One, Vol. 9, No. 1, e86627, 29.01.2014.

Research output: Contribution to journalArticle

Testini, M, Gurrado, A, Portincasa, P, Scacco, S, Marzullo, A, Piccinni, G, Lissidini, G, Greco, L, De Salvia, MA, Bonfrate, L, Debellis, L, Sardaro, N, Staffieri, F, Carratù, MR & Crovace, A 2014, 'Bovine pericardium patch wrapping intestinal anastomosis improves healing process and prevents leakage in a pig model', PLoS One, vol. 9, no. 1, e86627. https://doi.org/10.1371/journal.pone.0086627
Testini, Mario ; Gurrado, Angela ; Portincasa, Piero ; Scacco, Salvatore ; Marzullo, Andrea ; Piccinni, Giuseppe ; Lissidini, Germana ; Greco, Luigi ; De Salvia, Maria Antonietta ; Bonfrate, Leonilde ; Debellis, Lucantonio ; Sardaro, Nicola ; Staffieri, Francesco ; Carratù, Maria Rosaria ; Crovace, Antonio. / Bovine pericardium patch wrapping intestinal anastomosis improves healing process and prevents leakage in a pig model. In: PLoS One. 2014 ; Vol. 9, No. 1.
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