TY - JOUR
T1 - Bradykinin- and lipopolysaccharide-induced bradykinin B2 receptor expression, interleukin 8 release and nitrosative stress in bronchial epithelial cells BEAS-2B
T2 - Role for neutrophils
AU - Ricciardolo, Fabio L M
AU - Sorbello, Valentina
AU - Benedetto, Sabrina
AU - Defilippi, Ilaria
AU - Sabatini, Federica
AU - Robotti, Andrea
AU - Van Renswouw, Dein C.
AU - Bucca, Caterina
AU - Folkerts, Gert
AU - De Rose, Virginia
PY - 2012/11/5
Y1 - 2012/11/5
N2 - Bradykinin-induced interleukin (IL)-8 release should potentially activate neutrophils releasing myeloperoxidase (MPO) and subsequently generating nitrosative stress. We studied bradykinin-induced expression of bradykinin B2 receptor and bradykinin- and lipopolysaccharide (LPS)-induced IL-8 release, MPO (marker of neutrophil activation) and 3-nitrotyrosine (3-NT; marker of nitrosative stress) production in human bronchial epithelial cells BEAS-2B alone or in co-culture with human neutrophils. We evaluated B 2 receptor protein expression in BEAS-2B cells by immunostainings and Western blot analysis, and measured respectively bradykinin- or LPS-induced IL-8 release in BEAS-2B cells and bradykinin- and/or LPS-induced MPO and 3-NT production in BEAS-2B cells co-cultured with human neutrophils by ELISA. In addition, we evaluated bradykinin- and/or LPS-induced 3-NT formation in BEAS-2B cells co-cultured with human neutrophils by immunocytochemistry. Bradykinin up-regulates B2 receptor expression (P2 receptor antagonist HOE 140 or the selective bradykinin B 1 receptor antagonist Lys-(des-Arg9, Leu 8)-bradykinin alone halved IL-8 release and the combination of both drugs suppressed this effect. In BEAS-2B cells co-cultured with human neutrophils bradykinin increased MPO release and 3-NT production compared to BEAS-2B cells with human neutrophils (P
AB - Bradykinin-induced interleukin (IL)-8 release should potentially activate neutrophils releasing myeloperoxidase (MPO) and subsequently generating nitrosative stress. We studied bradykinin-induced expression of bradykinin B2 receptor and bradykinin- and lipopolysaccharide (LPS)-induced IL-8 release, MPO (marker of neutrophil activation) and 3-nitrotyrosine (3-NT; marker of nitrosative stress) production in human bronchial epithelial cells BEAS-2B alone or in co-culture with human neutrophils. We evaluated B 2 receptor protein expression in BEAS-2B cells by immunostainings and Western blot analysis, and measured respectively bradykinin- or LPS-induced IL-8 release in BEAS-2B cells and bradykinin- and/or LPS-induced MPO and 3-NT production in BEAS-2B cells co-cultured with human neutrophils by ELISA. In addition, we evaluated bradykinin- and/or LPS-induced 3-NT formation in BEAS-2B cells co-cultured with human neutrophils by immunocytochemistry. Bradykinin up-regulates B2 receptor expression (P2 receptor antagonist HOE 140 or the selective bradykinin B 1 receptor antagonist Lys-(des-Arg9, Leu 8)-bradykinin alone halved IL-8 release and the combination of both drugs suppressed this effect. In BEAS-2B cells co-cultured with human neutrophils bradykinin increased MPO release and 3-NT production compared to BEAS-2B cells with human neutrophils (P
KW - Bradykinin
KW - Bradykinin B receptor
KW - Bronchial epithelium
KW - Interleukin-8
KW - Lipopolysaccharide
KW - Nitrosative stress
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U2 - 10.1016/j.ejphar.2012.07.051
DO - 10.1016/j.ejphar.2012.07.051
M3 - Article
C2 - 22935637
AN - SCOPUS:84867334556
VL - 694
SP - 30
EP - 38
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 1-3
ER -