Bradykinin- and lipopolysaccharide-induced bradykinin B2 receptor expression, interleukin 8 release and nitrosative stress in bronchial epithelial cells BEAS-2B: Role for neutrophils

Fabio L M Ricciardolo, Valentina Sorbello, Sabrina Benedetto, Ilaria Defilippi, Federica Sabatini, Andrea Robotti, Dein C. Van Renswouw, Caterina Bucca, Gert Folkerts, Virginia De Rose

Research output: Contribution to journalArticlepeer-review

Abstract

Bradykinin-induced interleukin (IL)-8 release should potentially activate neutrophils releasing myeloperoxidase (MPO) and subsequently generating nitrosative stress. We studied bradykinin-induced expression of bradykinin B2 receptor and bradykinin- and lipopolysaccharide (LPS)-induced IL-8 release, MPO (marker of neutrophil activation) and 3-nitrotyrosine (3-NT; marker of nitrosative stress) production in human bronchial epithelial cells BEAS-2B alone or in co-culture with human neutrophils. We evaluated B 2 receptor protein expression in BEAS-2B cells by immunostainings and Western blot analysis, and measured respectively bradykinin- or LPS-induced IL-8 release in BEAS-2B cells and bradykinin- and/or LPS-induced MPO and 3-NT production in BEAS-2B cells co-cultured with human neutrophils by ELISA. In addition, we evaluated bradykinin- and/or LPS-induced 3-NT formation in BEAS-2B cells co-cultured with human neutrophils by immunocytochemistry. Bradykinin up-regulates B2 receptor expression (P2 receptor antagonist HOE 140 or the selective bradykinin B 1 receptor antagonist Lys-(des-Arg9, Leu 8)-bradykinin alone halved IL-8 release and the combination of both drugs suppressed this effect. In BEAS-2B cells co-cultured with human neutrophils bradykinin increased MPO release and 3-NT production compared to BEAS-2B cells with human neutrophils (P

Original languageEnglish
Pages (from-to)30-38
Number of pages9
JournalEuropean Journal of Pharmacology
Volume694
Issue number1-3
DOIs
Publication statusPublished - Nov 5 2012

Keywords

  • Bradykinin
  • Bradykinin B receptor
  • Bronchial epithelium
  • Interleukin-8
  • Lipopolysaccharide
  • Nitrosative stress

ASJC Scopus subject areas

  • Pharmacology

Fingerprint Dive into the research topics of 'Bradykinin- and lipopolysaccharide-induced bradykinin B2 receptor expression, interleukin 8 release and nitrosative stress in bronchial epithelial cells BEAS-2B: Role for neutrophils'. Together they form a unique fingerprint.

Cite this