Bradykinin-induced amyloid precursor protein secretion: A protein kinase C-independent mechanism that is not altered in fibroblasts from patients with sporadic Alzheimer's disease

Marco Racchi, Paola Ianna, Giuliano Binetti, Marco Trabucchi, Stefano Govoni

Research output: Contribution to journalArticlepeer-review

Abstract

We treated human skin fibroblasts with bradykinin (BK) and observed a concentration-dependent increase in the release of soluble amyloid precursor protein (sAPP). The estimated EC50 for the observed effect is 2.8 nM, which is of the same order of magnitude as the reported K(d) of BK binding in human skin fibroblasts. The effect of BK on sAPP secretion appears to be dependent on interaction of the ligand with the B2 type of BK receptors but independent of activation of protein kinase C. We also show that sAPP release after BK treatment in fibroblasts from patients with sporadic Alzheimer's disease is not different from control cells and is paralleled by equivalent levels of inositol trisphosphate production. A discussion of the differences from previously published work focuses on the possible divergent alterations in transduction systems in fibroblasts from patients with familial and sporadic Alzheimer's disease. Our results are the first example of receptor-mediated sAPP release in human skin fibroblasts and the first demonstration of the co-existence of protein kinase C-dependent and -independent mechanisms in these cells.

Original languageEnglish
Pages (from-to)1271-1275
Number of pages5
JournalBiochemical Journal
Volume330
Issue number3
Publication statusPublished - Mar 15 1998

ASJC Scopus subject areas

  • Biochemistry

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