BRAF and PIK3CA genes are somatically mutated in hepatocellular carcinoma among patients from South Italy

M. Colombino, P. Sperlongano, F. Izzo, F. Tatangelo, G. Botti, A. Lombardi, M. Accardo, L. Tarantino, I. Sordelli, M. Agresti, A. Abbruzzese, M. Caraglia, G. Palmieri

Research output: Contribution to journalArticlepeer-review

Abstract

Poor data have been previously reported about the mutation rates in K-RAS, BRAF, and PIK3CA genes among patients with hepatocellular carcinoma (HCC). Here we further elucidated the role of these genes in pathogenesis of primary hepatic malignancies. Archival tumour tissue from 65 HCC patients originating from South Italy were screened for mutations in these candidate genes by direct sequencing. Overall, oncogenic mutations were detected in 15 (23%) patients for BRAF gene, 18 (28%) for PIK3CA gene, and 1 (2%) for K-RAS gene. Using statistical analysis, BRAF mutations were significantly correlated with the presence of either multiple HCC nodules (P=0.021) or higher proliferation rates (P=0.034). Although further extensive screenings are awaited in HCC patients among different populations, our findings clearly indicated that mutational activation of both BRAF and PIK3CA genes does contribute to hepatocellular tumorigenesis at somatic level in Southern Italian population.

Original languageEnglish
Article numbere259
JournalCell Death and Disease
Volume3
Issue number1
DOIs
Publication statusPublished - Jan 2012

Keywords

  • Cancer genes
  • Hepatocellular carcinoma
  • Mutation analysis
  • Patients' molecular classification

ASJC Scopus subject areas

  • Cell Biology
  • Immunology
  • Cancer Research
  • Cellular and Molecular Neuroscience
  • Medicine(all)

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