TY - JOUR
T1 - BRAF and PIK3CA genes are somatically mutated in hepatocellular carcinoma among patients from South Italy
AU - Colombino, M.
AU - Sperlongano, P.
AU - Izzo, F.
AU - Tatangelo, F.
AU - Botti, G.
AU - Lombardi, A.
AU - Accardo, M.
AU - Tarantino, L.
AU - Sordelli, I.
AU - Agresti, M.
AU - Abbruzzese, A.
AU - Caraglia, M.
AU - Palmieri, G.
PY - 2012/1
Y1 - 2012/1
N2 - Poor data have been previously reported about the mutation rates in K-RAS, BRAF, and PIK3CA genes among patients with hepatocellular carcinoma (HCC). Here we further elucidated the role of these genes in pathogenesis of primary hepatic malignancies. Archival tumour tissue from 65 HCC patients originating from South Italy were screened for mutations in these candidate genes by direct sequencing. Overall, oncogenic mutations were detected in 15 (23%) patients for BRAF gene, 18 (28%) for PIK3CA gene, and 1 (2%) for K-RAS gene. Using statistical analysis, BRAF mutations were significantly correlated with the presence of either multiple HCC nodules (P=0.021) or higher proliferation rates (P=0.034). Although further extensive screenings are awaited in HCC patients among different populations, our findings clearly indicated that mutational activation of both BRAF and PIK3CA genes does contribute to hepatocellular tumorigenesis at somatic level in Southern Italian population.
AB - Poor data have been previously reported about the mutation rates in K-RAS, BRAF, and PIK3CA genes among patients with hepatocellular carcinoma (HCC). Here we further elucidated the role of these genes in pathogenesis of primary hepatic malignancies. Archival tumour tissue from 65 HCC patients originating from South Italy were screened for mutations in these candidate genes by direct sequencing. Overall, oncogenic mutations were detected in 15 (23%) patients for BRAF gene, 18 (28%) for PIK3CA gene, and 1 (2%) for K-RAS gene. Using statistical analysis, BRAF mutations were significantly correlated with the presence of either multiple HCC nodules (P=0.021) or higher proliferation rates (P=0.034). Although further extensive screenings are awaited in HCC patients among different populations, our findings clearly indicated that mutational activation of both BRAF and PIK3CA genes does contribute to hepatocellular tumorigenesis at somatic level in Southern Italian population.
KW - Cancer genes
KW - Hepatocellular carcinoma
KW - Mutation analysis
KW - Patients' molecular classification
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U2 - 10.1038/cddis.2011.136
DO - 10.1038/cddis.2011.136
M3 - Article
C2 - 22258409
AN - SCOPUS:84856845687
VL - 3
JO - Cell Death and Disease
JF - Cell Death and Disease
SN - 2041-4889
IS - 1
M1 - e259
ER -