TY - JOUR
T1 - Brain malformations in the sheep model of myelomeningocele are similar to those found in human disease
T2 - Preliminary report
AU - Encinas Hernández, Jose Luis
AU - Soto, C.
AU - García-Cabezas, M. A.
AU - Pederiva, F.
AU - Garriboli, M.
AU - Rodríguez, R.
AU - Peiró, J. L.
AU - Carceller, F.
AU - López-Santamaría, M.
AU - Tovar, J. A.
PY - 2008/12
Y1 - 2008/12
N2 - Purpose: To examine if brain malformations, similar to those which account for cognitive disorders seen in human disease, are present in an ovine model of myelomeningocele (MMC). Methods: An MMC-like lesion was surgically created in 16 fetal lambs between 60 and 80 days of gestation. Ten did not undergo fetal repair (group A), 2 were repaired with an open two-layer closure (group B), 2 with open bioglue coverage (group C) and 2 with fetoscopic coverage (group D). Lambs were killed and their brains were examined. Two brains from normal unoperated lambs served as controls. Results: Thirteen lambs died in utero (81%). Two lambs in group A and 1 in group B were delivered at term. Group A brains showed hydrocephalus and extensive areas of polymicrogyria. There was also an extensive denudation of the ependymal lining under the polymicrogyric areas and the corpus callosum was thinner than normal. No hindbrain herniation was observed. Brains from group B and the control did not show any of these abnormalities. Conclusions: Some of the central nervous system abnormalities associated to MMC in human patients are also found in the uncorrected fetal lamb model of MMC but not in the only survivor to intrauterine coverage. Further studies are necessary to ascertain if these abnormalities can be prevented by coverage of the defect.
AB - Purpose: To examine if brain malformations, similar to those which account for cognitive disorders seen in human disease, are present in an ovine model of myelomeningocele (MMC). Methods: An MMC-like lesion was surgically created in 16 fetal lambs between 60 and 80 days of gestation. Ten did not undergo fetal repair (group A), 2 were repaired with an open two-layer closure (group B), 2 with open bioglue coverage (group C) and 2 with fetoscopic coverage (group D). Lambs were killed and their brains were examined. Two brains from normal unoperated lambs served as controls. Results: Thirteen lambs died in utero (81%). Two lambs in group A and 1 in group B were delivered at term. Group A brains showed hydrocephalus and extensive areas of polymicrogyria. There was also an extensive denudation of the ependymal lining under the polymicrogyric areas and the corpus callosum was thinner than normal. No hindbrain herniation was observed. Brains from group B and the control did not show any of these abnormalities. Conclusions: Some of the central nervous system abnormalities associated to MMC in human patients are also found in the uncorrected fetal lamb model of MMC but not in the only survivor to intrauterine coverage. Further studies are necessary to ascertain if these abnormalities can be prevented by coverage of the defect.
KW - Brain
KW - Development
KW - Fetal
KW - Myelomeningocele
KW - Sheep
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U2 - 10.1007/s00383-008-2276-8
DO - 10.1007/s00383-008-2276-8
M3 - Article
C2 - 18989683
AN - SCOPUS:56649112833
VL - 24
SP - 1335
EP - 1340
JO - Pediatric Surgery International
JF - Pediatric Surgery International
SN - 0179-0358
IS - 12
ER -