Brain Oxidative Damage Following Acute Immobilization and Mild Emotional Stress

Fulvio Marzatico, Laura Bertorelli, Orietta Pansarasa, Paola Guallini, Carla Torri, Giuseppe Biagini

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We studied the role of free radicals on brain oxidative damage in rats after acute immobilization stress (restraint) and mild emotional stress (handling). To investigate brain oxidative damage, CuZn and Mn dependent superoxide dismutase (CuZn SOD, Mn SOD) activities, lipid peroxidation (TBARs), Na+K+ ATPase activity, protein carbonyl (PrC), and reduced and oxidized glutathione (GSH, GSSG) levels were measured in the cerebral cortex (CTX), hippocampus (HIP), and striatum (ST) of the animals after the two different stress stimuli. Because stress produces abnormalities in the hypothalamic-pituitary-adrenal axis, the intensity of the two stress conditions were measured by plasmatic corticosteroid (COR) levels: particularly, COR levels doubled in handled rats and increased 15-fold in restrained animals. The SOD activities increased in CTX and decreased in HIP of the handled rats, while in ST a significant decrease in handled animals but an increase in restrained animals occurred. TBARs, GSH, and GSSG levels remained unchanged, while an index of glutathione redox decreased significantly in ST of handled animals and in CTX of restrained ones. Na+K+ ATPase activity increased significantly in the HIP and ST of both groups of stressed rats. The stress induced a remarkable increase in PrC levels in all studied cerebral areas. These findings provide evidence to support the idea that stress produces oxidants but that the oxidative damage in stress differs in cerebral areas and could contribute to the degenerative mechanism of aging.

Original languageEnglish
Pages (from-to)223-236
Number of pages14
JournalInternational Journal of Stress Management
Volume5
Issue number4
Publication statusPublished - 1998

Fingerprint

brain damage
Psychological Stress
Immobilization
Glutathione Disulfide
Brain
Hippocampus
animal
Thiobarbituric Acid Reactive Substances
Superoxide Dismutase
Glutathione
Adrenal Cortex Hormones
Oxidants
Cerebral Cortex
Lipid Peroxidation
Oxidation-Reduction
Free Radicals
Proteins
Damage
Emotion
stimulus

Keywords

  • Antioxidant enzymes
  • Brain
  • Emotional stress
  • Glutathione
  • Oxidative damage
  • Protein oxidation

ASJC Scopus subject areas

  • Business, Management and Accounting(all)
  • Psychology(all)
  • Applied Psychology

Cite this

Marzatico, F., Bertorelli, L., Pansarasa, O., Guallini, P., Torri, C., & Biagini, G. (1998). Brain Oxidative Damage Following Acute Immobilization and Mild Emotional Stress. International Journal of Stress Management, 5(4), 223-236.

Brain Oxidative Damage Following Acute Immobilization and Mild Emotional Stress. / Marzatico, Fulvio; Bertorelli, Laura; Pansarasa, Orietta; Guallini, Paola; Torri, Carla; Biagini, Giuseppe.

In: International Journal of Stress Management, Vol. 5, No. 4, 1998, p. 223-236.

Research output: Contribution to journalArticle

Marzatico, F, Bertorelli, L, Pansarasa, O, Guallini, P, Torri, C & Biagini, G 1998, 'Brain Oxidative Damage Following Acute Immobilization and Mild Emotional Stress', International Journal of Stress Management, vol. 5, no. 4, pp. 223-236.
Marzatico, Fulvio ; Bertorelli, Laura ; Pansarasa, Orietta ; Guallini, Paola ; Torri, Carla ; Biagini, Giuseppe. / Brain Oxidative Damage Following Acute Immobilization and Mild Emotional Stress. In: International Journal of Stress Management. 1998 ; Vol. 5, No. 4. pp. 223-236.
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