Brain protein preservation largely depends on the postmortem storage temperature: Implications for study of proteins in human neurologic diseases and management of brain banks: A BrainNet Europe study

Isidre Ferrer, Gabriel Santpere, Thomas Arzberger, Jeanne Bell, Rosa Blanco, Susana Boluda, Herbert Budka, Margarita Carmona, Giorgio Giaccone, Bjarne Krebs, Lucia Limido, Piero Parchi, Berta Puig, Rosaria Strammiello, Thomas Ströbel, Hans Kretzschmar

Research output: Contribution to journalArticlepeer-review

Abstract

The present study was designed to reveal protein modifications in control cases related with postmortem delay and temperature of storage in 3 paradigms in which the same postmortem tissue sample (frontal cortex) was frozen a short time after death or stored at 1°C, 4°C, or room temperature and then frozen at -80°C at different intervals. No evidence of protein degradation as revealed with monodimensional gel electrophoresis and Western blotting was observed in samples artificially stored at 1°C and then frozen at different intervals up to 50 hours after death. However, the levels of several proteins were modified in samples stored at 4°C and this effect was more marked in samples stored at room temperature. Two-dimensional gel electrophoresis and mass spectrometry further corroborated these observations and permitted the identification of other proteins vulnerable or resistant to postmortem delay. Finally, gel electrophoresis and Western blotting of sarkosyl-insoluble fractions in Alzheimer disease showed reduced intensity of phospho-tau-specific bands with postmortem delay with the effects being more dramatic when the brain samples were stored at room temperature for long periods. These results emphasize the necessity of reducing the body temperature after death to minimize protein degradation.

Original languageEnglish
Pages (from-to)35-46
Number of pages12
JournalJournal of Neuropathology and Experimental Neurology
Volume66
Issue number1
DOIs
Publication statusPublished - Jan 2007

Keywords

  • Brain banks
  • Human brain tissue
  • Postmortem delay
  • Protein preservation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neuroscience(all)

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