BRCA1, PARP1 and γH2AX in acute myeloid leukemia: Role as biomarkers of response to the PARP inhibitor olaparib

Isabella Faraoni, Mirco Compagnone, Serena Lavorgna, Daniela Francesca Angelini, Maria Teresa Cencioni, Eleonora Piras, Paola Panetta, Tiziana Ottone, Susanna Dolci, Adriano Venditti, Grazia Graziani, Francesco Lo-Coco

Research output: Contribution to journalArticlepeer-review


Olaparib (AZD-2281, Ku-0059436) is an orally bioavailable and well-tolerated poly(ADP-ribose) polymerase (PARP) inhibitor currently under investigation in patients with solid tumors. To study the clinical potential of olaparib as a single-agent for the treatment of acute myeloid leukemia (AML) patients, we analyzed the in vitro sensitivity of AML cell lines and primary blasts. Clinically achievable concentrations of olaparib were able to induce cell death in the majority of primary AML case samples (88%) and tested cell lines. At these concentrations, olaparib preferentially killed leukemic blasts sparing normal lymphocytes derived from the same patient and did not substantially affect the viability of normal bone marrow and CD34-enriched peripheral blood cells obtained from healthy donors. Most primary AML analyzed were characterized by low BRCA1 mRNA level and undetectable protein expression that likely contributed to explain their sensitivity to olaparib. Noteworthy, while PARP1 over-expression was detected in blasts not responsive to olaparib, phosphorylation of the histone H2AFX (γH2AX) was associated with drug sensitivity. As to genetic features of tested cases the highest sensitivity was shown by a patient carrying a 11q23 deletion. The high sensitivity of AML blasts and the identification of biomarkers potentially able to predict response and/or resistance may foster further investigation of olaparib monotherapy for AML patients unfit to conventional chemotherapy.

Original languageEnglish
Pages (from-to)462-472
Number of pages11
JournalBiochimica et Biophysica Acta - Molecular Basis of Disease
Issue number3
Publication statusPublished - Mar 1 2015


  • Acute myeloid leukemia
  • BRCA1
  • H2AX
  • Olaparib
  • PARP inhibitor
  • PARP1

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Medicine(all)


Dive into the research topics of 'BRCA1, PARP1 and γH2AX in acute myeloid leukemia: Role as biomarkers of response to the PARP inhibitor olaparib'. Together they form a unique fingerprint.

Cite this