Brief report: Peptide products of the neurotrophin-inducible gene vgf are produced in human neuroendocrine cells from early development and increase in hyperplasia and neoplasia

Guido Rindi, Lisa Licini, Vittorio Necchi, Lorena Bottarelli, Nicoletta Campanini, Cinzia Azzoni, Maurizio Favret, Giovanna Giordano, Filomena D'Amato, Carla Brancia, Enrico Solcia, Gian Luca Ferri

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background: Although the neurotrophin-inducible gene vgf is expressed in mammalian neurons and endocrine cells, limited data is available in man. Aim: The objective of the study was to map proVGF peptides in human endocrine cells during development, adulthood, hyperplasia, and tumors. Methods: Antisera were generated against peptides related to internal cleavage or cleavage-amidation sites (rat proVGF422-430 and human proVGF298-306-NH2) and the proVGF C-terminal ending (human proVGF607-615). Developing and normal adult endocrine cells, hyperplastic endocrine lesions (thyroid, parathyroid, lung, and stomach), and 120 tumors (102 endocrine) were studied. Immunogold electron microscopy was performed on normal adult pancreas and gut, and Western blotting was performed on extracts of control tissues and endocrine tumors. Results: proVGF fragments were revealed in developing pituitary, gut, pancreas, and adrenal medulla from 10 gestational weeks, in normal adult pituitary and adrenal medulla, pancreatic glucagon, and insulin cells and gut serotonin cells, in hyperplastic thyroid calcitonin cells, lung P cells, gastric enterochromaffin-like cells, and gastrin cells, and in 88 of 102 endocrine tumors. At electron microscopy proVGF immunoreactivity was restricted to electron-dense granules. Western blotting revealed large molecular weight forms and cleavage fragments in both control tissues and tumor extracts. Conclusions: proVGF-related peptides are present in endocrine cells early during development and adulthood and increase in hyperplasia and tumors, and proVGF fragments could be novel diagnostic tools for endocrine cells and related lesions, including tumors.

Original languageEnglish
Pages (from-to)2811-2815
Number of pages5
JournalJournal of Clinical Endocrinology and Metabolism
Volume92
Issue number7
DOIs
Publication statusPublished - Jul 2007

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Neuroendocrine Cells
Nerve Growth Factors
Hyperplasia
Tumors
Endocrine Cells
Genes
Peptides
Cells
Neoplasms
Adrenal Medulla
Tissue Extracts
Electron microscopy
Pancreas
Stomach
Electron Microscopy
Thyroid Gland
Western Blotting
Enterochromaffin-like Cells
Tissue
Lung

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Brief report : Peptide products of the neurotrophin-inducible gene vgf are produced in human neuroendocrine cells from early development and increase in hyperplasia and neoplasia. / Rindi, Guido; Licini, Lisa; Necchi, Vittorio; Bottarelli, Lorena; Campanini, Nicoletta; Azzoni, Cinzia; Favret, Maurizio; Giordano, Giovanna; D'Amato, Filomena; Brancia, Carla; Solcia, Enrico; Ferri, Gian Luca.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 92, No. 7, 07.2007, p. 2811-2815.

Research output: Contribution to journalArticle

Rindi, Guido ; Licini, Lisa ; Necchi, Vittorio ; Bottarelli, Lorena ; Campanini, Nicoletta ; Azzoni, Cinzia ; Favret, Maurizio ; Giordano, Giovanna ; D'Amato, Filomena ; Brancia, Carla ; Solcia, Enrico ; Ferri, Gian Luca. / Brief report : Peptide products of the neurotrophin-inducible gene vgf are produced in human neuroendocrine cells from early development and increase in hyperplasia and neoplasia. In: Journal of Clinical Endocrinology and Metabolism. 2007 ; Vol. 92, No. 7. pp. 2811-2815.
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abstract = "Background: Although the neurotrophin-inducible gene vgf is expressed in mammalian neurons and endocrine cells, limited data is available in man. Aim: The objective of the study was to map proVGF peptides in human endocrine cells during development, adulthood, hyperplasia, and tumors. Methods: Antisera were generated against peptides related to internal cleavage or cleavage-amidation sites (rat proVGF422-430 and human proVGF298-306-NH2) and the proVGF C-terminal ending (human proVGF607-615). Developing and normal adult endocrine cells, hyperplastic endocrine lesions (thyroid, parathyroid, lung, and stomach), and 120 tumors (102 endocrine) were studied. Immunogold electron microscopy was performed on normal adult pancreas and gut, and Western blotting was performed on extracts of control tissues and endocrine tumors. Results: proVGF fragments were revealed in developing pituitary, gut, pancreas, and adrenal medulla from 10 gestational weeks, in normal adult pituitary and adrenal medulla, pancreatic glucagon, and insulin cells and gut serotonin cells, in hyperplastic thyroid calcitonin cells, lung P cells, gastric enterochromaffin-like cells, and gastrin cells, and in 88 of 102 endocrine tumors. At electron microscopy proVGF immunoreactivity was restricted to electron-dense granules. Western blotting revealed large molecular weight forms and cleavage fragments in both control tissues and tumor extracts. Conclusions: proVGF-related peptides are present in endocrine cells early during development and adulthood and increase in hyperplasia and tumors, and proVGF fragments could be novel diagnostic tools for endocrine cells and related lesions, including tumors.",
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T2 - Peptide products of the neurotrophin-inducible gene vgf are produced in human neuroendocrine cells from early development and increase in hyperplasia and neoplasia

AU - Rindi, Guido

AU - Licini, Lisa

AU - Necchi, Vittorio

AU - Bottarelli, Lorena

AU - Campanini, Nicoletta

AU - Azzoni, Cinzia

AU - Favret, Maurizio

AU - Giordano, Giovanna

AU - D'Amato, Filomena

AU - Brancia, Carla

AU - Solcia, Enrico

AU - Ferri, Gian Luca

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N2 - Background: Although the neurotrophin-inducible gene vgf is expressed in mammalian neurons and endocrine cells, limited data is available in man. Aim: The objective of the study was to map proVGF peptides in human endocrine cells during development, adulthood, hyperplasia, and tumors. Methods: Antisera were generated against peptides related to internal cleavage or cleavage-amidation sites (rat proVGF422-430 and human proVGF298-306-NH2) and the proVGF C-terminal ending (human proVGF607-615). Developing and normal adult endocrine cells, hyperplastic endocrine lesions (thyroid, parathyroid, lung, and stomach), and 120 tumors (102 endocrine) were studied. Immunogold electron microscopy was performed on normal adult pancreas and gut, and Western blotting was performed on extracts of control tissues and endocrine tumors. Results: proVGF fragments were revealed in developing pituitary, gut, pancreas, and adrenal medulla from 10 gestational weeks, in normal adult pituitary and adrenal medulla, pancreatic glucagon, and insulin cells and gut serotonin cells, in hyperplastic thyroid calcitonin cells, lung P cells, gastric enterochromaffin-like cells, and gastrin cells, and in 88 of 102 endocrine tumors. At electron microscopy proVGF immunoreactivity was restricted to electron-dense granules. Western blotting revealed large molecular weight forms and cleavage fragments in both control tissues and tumor extracts. Conclusions: proVGF-related peptides are present in endocrine cells early during development and adulthood and increase in hyperplasia and tumors, and proVGF fragments could be novel diagnostic tools for endocrine cells and related lesions, including tumors.

AB - Background: Although the neurotrophin-inducible gene vgf is expressed in mammalian neurons and endocrine cells, limited data is available in man. Aim: The objective of the study was to map proVGF peptides in human endocrine cells during development, adulthood, hyperplasia, and tumors. Methods: Antisera were generated against peptides related to internal cleavage or cleavage-amidation sites (rat proVGF422-430 and human proVGF298-306-NH2) and the proVGF C-terminal ending (human proVGF607-615). Developing and normal adult endocrine cells, hyperplastic endocrine lesions (thyroid, parathyroid, lung, and stomach), and 120 tumors (102 endocrine) were studied. Immunogold electron microscopy was performed on normal adult pancreas and gut, and Western blotting was performed on extracts of control tissues and endocrine tumors. Results: proVGF fragments were revealed in developing pituitary, gut, pancreas, and adrenal medulla from 10 gestational weeks, in normal adult pituitary and adrenal medulla, pancreatic glucagon, and insulin cells and gut serotonin cells, in hyperplastic thyroid calcitonin cells, lung P cells, gastric enterochromaffin-like cells, and gastrin cells, and in 88 of 102 endocrine tumors. At electron microscopy proVGF immunoreactivity was restricted to electron-dense granules. Western blotting revealed large molecular weight forms and cleavage fragments in both control tissues and tumor extracts. Conclusions: proVGF-related peptides are present in endocrine cells early during development and adulthood and increase in hyperplasia and tumors, and proVGF fragments could be novel diagnostic tools for endocrine cells and related lesions, including tumors.

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