Broadening the horizon - Level 2.5 of the HUPO-PSI format for molecular interactions

Samuel Kerrien, Sandra Orchard, Luisa Montecchi-Palazzi, Bruno Aranda, Antony F. Quinn, Nisha Vinod, Gary D. Bader, Ioannis Xenarios, Jérôme Wojcik, David Sherman, Mike Tyers, John J. Salama, Susan Moore, Arnaud Ceol, Andrew Chatr-Aryamontri, Matthias Oesterheld, Volker Stümpflen, Lukasz Salwinski, Jason Nerothin, Ethan CeramiMichael E. Cusick, Marc Vidal, Michael Gilson, John Armstrong, Peter Woollard, Christopher Hogue, David Eisenberg, Gianni Cesareni, Rolf Apweiler, Henning Hermjakob

Research output: Contribution to journalArticlepeer-review


Background: Molecular interaction Information is a key resource in modern biomedical research. Publicly available data have previously been provided in a broad array of diverse formats, making access to this very difficult. The publication and wide implementation of the Human Proteome Organisation Proteomics Standards Initiative Molecular Interactions (HUPO PSI-MI) format in 2004 was a major step towards the establishment of a single, unified format by which molecular interactions should be presented, but focused purely on protein-protein interactions. Results: The HUPO-PSI has further developed the PSI-MI XML schema to enable the description of interactions between a wider range of molecular types, for example nucleic acids, chemical entities, and molecular complexes. Extensive details about each supported molecular interaction can now be captured, including the biological role of each molecule within that interaction, detailed description of interacting domains, and the kinetic parameters of the interaction. The format is supported by data management and analysis tools and has been adopted by major interaction data providers. Additionally, a simpler, tab-delimited format MITAB2.5 has been developed for the benefit of users who require only minimal information in an easy to access configuration. Conclusion: The PSI-MI XML2.5 and MITAB2.5 formats have been jointly developed by interaction data producers and providers from both the academic and commercial sector, and are already widely implemented and well supported by an active development community. PSI-MI XML2.5 enables the description of highly detailed molecular interaction data and facilitates data exchange between databases and users without loss of information. MITAB2.5 is a simpler format appropriate for fast Perl parsing or loading into Microsoft Excel.

Original languageEnglish
Article number44
JournalBMC Biology
Publication statusPublished - Oct 9 2007

ASJC Scopus subject areas

  • Biotechnology
  • Structural Biology
  • Developmental Biology
  • Physiology
  • Plant Science
  • Cell Biology
  • Ecology, Evolution, Behavior and Systematics


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