Btg2 enhances retinoic acid-induced differentiation by modulating histone H4 methylation and acetylation

Daniela Passeri, Antonella Marcucci, Giovanni Rizzo, Monia Billi, Maddalena Panigada, Luca Leonardi, Felice Tirone, Francesco Grignani

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Retinoic acid controls hematopoietic differentiation through the transcription factor activity of its receptors. They act on specific target genes by recruiting protein complexes that deacetylate or acetylate histones and modify chromatin status. The regulation of this process is affected by histone methyltransferases, which can inhibit or activate transcription depending on their amino acid target. We show here that retinoic acid treatment of hematopoietic cells induces the expression of BTG2. Overexpression of this protein increases RARα transcriptional activity and the differentiation response to retinoic acid of myeloid leukemia cells and CD34+ hematopoietic progenitors. In the absence of retinoic acid, BTG2 is present in the RARα transcriptional complex, together with the arginine methyltransferase PRMT1 and Sin3A. Overexpressed BTG2 increases PRMT1 participation in the RARα protein complex on the RARβ promoter, a target gene model, and enhances gene-specific histone H4 arginine methylation. Upon RA treatment Sin3A, BTG2, and PRMT1 detach from RARα and thereafter BGT2 and PRMT1 are driven to the cytoplasm. These events prime histone H4 demethylation and acetylation. Overall, our data show that BTG2 contributes to retinoic acid activity by favoring differentiation through a gene-specific modification of histone H4 arginine methylation and acetylation levels.

Original languageEnglish
Pages (from-to)5023-5032
Number of pages10
JournalMolecular and Cellular Biology
Volume26
Issue number13
DOIs
Publication statusPublished - Jul 2006

Fingerprint

Acetylation
Tretinoin
Histones
Methylation
Arginine
Genes
Proteins
Myeloid Leukemia
Myeloid Cells
Chromatin
Cytoplasm
Transcription Factors
Amino Acids

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Passeri, D., Marcucci, A., Rizzo, G., Billi, M., Panigada, M., Leonardi, L., ... Grignani, F. (2006). Btg2 enhances retinoic acid-induced differentiation by modulating histone H4 methylation and acetylation. Molecular and Cellular Biology, 26(13), 5023-5032. https://doi.org/10.1128/MCB.01360-05

Btg2 enhances retinoic acid-induced differentiation by modulating histone H4 methylation and acetylation. / Passeri, Daniela; Marcucci, Antonella; Rizzo, Giovanni; Billi, Monia; Panigada, Maddalena; Leonardi, Luca; Tirone, Felice; Grignani, Francesco.

In: Molecular and Cellular Biology, Vol. 26, No. 13, 07.2006, p. 5023-5032.

Research output: Contribution to journalArticle

Passeri, D, Marcucci, A, Rizzo, G, Billi, M, Panigada, M, Leonardi, L, Tirone, F & Grignani, F 2006, 'Btg2 enhances retinoic acid-induced differentiation by modulating histone H4 methylation and acetylation', Molecular and Cellular Biology, vol. 26, no. 13, pp. 5023-5032. https://doi.org/10.1128/MCB.01360-05
Passeri, Daniela ; Marcucci, Antonella ; Rizzo, Giovanni ; Billi, Monia ; Panigada, Maddalena ; Leonardi, Luca ; Tirone, Felice ; Grignani, Francesco. / Btg2 enhances retinoic acid-induced differentiation by modulating histone H4 methylation and acetylation. In: Molecular and Cellular Biology. 2006 ; Vol. 26, No. 13. pp. 5023-5032.
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