Buffer effect on the kinetics of ornithine carbamyl transferase by HPLC

V. Carunchio, A. M. Girelli, A. Messina

Research output: Contribution to journalArticlepeer-review

Abstract

A kinetic study of ornithine carbamyl transferase (OCT) from Streptococcus faecalis has been carried out, employing a new HPLC method based on the direct determination of citrulline. A different kinetic pattern was observed in tris(hydroxymethyl)aminomethane (TRIS) and triethanolamine (TEA) buffers: a ping-pong or an ordered sequential mechanism are suggested, respectively. The inhibition of Tris buffer on OCT reaction has also been demonstrated. The inhibition of Tris buffer on OCT reaction has also been demonstrated.

Original languageEnglish
Pages (from-to)65-69
Number of pages5
JournalBiomedical Chromatography
Volume13
Issue number1
DOIs
Publication statusPublished - 1999

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Pharmacology

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