Buprenorphine inhibits bradykinin-induced release of calcitonin gene-related peptide from rat trigeminal neurons via both μ-opioid and nociceptin/orphanin peptide receptors

Alessandro Capuano, Alice De Corato, Giuseppe Tringali, Diego Currò, Cinzia Dello Russo, Pierluigi Navarra

Research output: Contribution to journalArticle


In this study we used the dual opioid and nociceptin/orphanin peptide (NOP) agonist buprenorphine to investigate the relative contributions of opioid and NOP systems in regulating bradykinin-stimulated calcitonin-gene related peptide (CGRP) release from primary cultures of neonatal rat trigeminal neurons. We found that: bradykinin stimulates CGRP secretion either by a direct effect or after applying so-called "bradykinin -priming" protocol. In both cases, buprenorphine was able to inhibit bradykinin-stimulated CGRP secretion; however, inhibition was mediated by NOP receptors when buprenorphine was added to the incubation medium along with bradykinin, whereas it appeared to be mediated by μ-opioid receptors in bradykinin priming experiments. Bradykinin treatments also caused an increase in neuronal prostaglandin production; prostanoids appeared to be involved in the stimulatory effects of bradykinin as well as in buprenorphine inhibition, through apparently unrelated mechanisms.

Original languageEnglish
Pages (from-to)45-50
Number of pages6
JournalEuropean Journal of Pharmacology
Issue number1-3
Publication statusPublished - May 1 2009



  • Bradykinin
  • Buprenorphine
  • Calcitonin gene-related peptide
  • NOP receptor
  • Rat trigeminal neuron

ASJC Scopus subject areas

  • Pharmacology

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