Burkholderia cepacia complex in cystic fibrosis and non-cystic fibrosis patients: Identification of a cluster of epidemic lineages

A. Agodi, M. Barchitta, V. Gianninò, A. Collura, T. Pensabene, M. L. Garlaschi, C. Pasquarella, F. Luzzaro, F. Sinatra, E. Mahenthiralingam, S. Stefani

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

This study was performed in order to compare Burkholderia cepacia complex strains from cystic fibrosis (CF) and non-CF patients at the genomovar, genetic and epidemiological levels. A total of 92 B. cepacia respiratory tract isolates were obtained from patients attending the following CF centres: Catania and Palermo, Sicily; Gualdo Tadino, Central Italy, and Milan, Northern Italy. A total of 23 B. cepacia isolates were obtained from blood, surgical wound, and intravenous catheter sources of patients without CF, hospitalized in Catania and Varese, Northern Italy. Genomovar status identification, clonality and genetic relatedness determination, antibiotic susceptibility pattern determination and electron microscopy were performed. Transmission of infection was shown in both CF and non-CF patients by identifying clonality of responsible strains. In total 13 clones were involved in cross-transmission episodes. No outbreak was described involving both CF and non-CF patients. The present study indicates the existence of a distinct cluster of strains responsible for epidemics in CF and non-CF patients, based on their genetic relatedness, distinct from strains associated with no or negligible transmissibility. This result suggests that transmissibility is not only associated with a specific genomovar in CF patients, but also with a group of genetically related lineages in CF and non-CF patients. A key role is shown for both segregation measures and careful surveillance of infection, based on selective culture, molecular identification and epidemiological characterization of individual isolates.

Original languageEnglish
Pages (from-to)188-195
Number of pages8
JournalJournal of Hospital Infection
Volume50
Issue number3
DOIs
Publication statusPublished - Mar 2002

Fingerprint

Burkholderia cepacia complex
Cystic Fibrosis
Fibrosis
Italy
Burkholderia cepacia
Sicily
Infectious Disease Transmission
Respiratory System
Disease Outbreaks
Electron Microscopy
Catheters
Clone Cells
Anti-Bacterial Agents

Keywords

  • Burkholderia cepacia
  • Cystic fibrosis
  • Epidemiology
  • Nosocomial infections

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Parasitology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

Cite this

Burkholderia cepacia complex in cystic fibrosis and non-cystic fibrosis patients : Identification of a cluster of epidemic lineages. / Agodi, A.; Barchitta, M.; Gianninò, V.; Collura, A.; Pensabene, T.; Garlaschi, M. L.; Pasquarella, C.; Luzzaro, F.; Sinatra, F.; Mahenthiralingam, E.; Stefani, S.

In: Journal of Hospital Infection, Vol. 50, No. 3, 03.2002, p. 188-195.

Research output: Contribution to journalArticle

Agodi, A, Barchitta, M, Gianninò, V, Collura, A, Pensabene, T, Garlaschi, ML, Pasquarella, C, Luzzaro, F, Sinatra, F, Mahenthiralingam, E & Stefani, S 2002, 'Burkholderia cepacia complex in cystic fibrosis and non-cystic fibrosis patients: Identification of a cluster of epidemic lineages', Journal of Hospital Infection, vol. 50, no. 3, pp. 188-195. https://doi.org/10.1053/jhin.2001.1160
Agodi, A. ; Barchitta, M. ; Gianninò, V. ; Collura, A. ; Pensabene, T. ; Garlaschi, M. L. ; Pasquarella, C. ; Luzzaro, F. ; Sinatra, F. ; Mahenthiralingam, E. ; Stefani, S. / Burkholderia cepacia complex in cystic fibrosis and non-cystic fibrosis patients : Identification of a cluster of epidemic lineages. In: Journal of Hospital Infection. 2002 ; Vol. 50, No. 3. pp. 188-195.
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