Abstract
This study was performed in order to compare Burkholderia cepacia complex strains from cystic fibrosis (CF) and non-CF patients at the genomovar, genetic and epidemiological levels. A total of 92 B. cepacia respiratory tract isolates were obtained from patients attending the following CF centres: Catania and Palermo, Sicily; Gualdo Tadino, Central Italy, and Milan, Northern Italy. A total of 23 B. cepacia isolates were obtained from blood, surgical wound, and intravenous catheter sources of patients without CF, hospitalized in Catania and Varese, Northern Italy. Genomovar status identification, clonality and genetic relatedness determination, antibiotic susceptibility pattern determination and electron microscopy were performed. Transmission of infection was shown in both CF and non-CF patients by identifying clonality of responsible strains. In total 13 clones were involved in cross-transmission episodes. No outbreak was described involving both CF and non-CF patients. The present study indicates the existence of a distinct cluster of strains responsible for epidemics in CF and non-CF patients, based on their genetic relatedness, distinct from strains associated with no or negligible transmissibility. This result suggests that transmissibility is not only associated with a specific genomovar in CF patients, but also with a group of genetically related lineages in CF and non-CF patients. A key role is shown for both segregation measures and careful surveillance of infection, based on selective culture, molecular identification and epidemiological characterization of individual isolates.
| Original language | English |
|---|---|
| Pages (from-to) | 188-195 |
| Number of pages | 8 |
| Journal | Journal of Hospital Infection |
| Volume | 50 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 2002 |
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Keywords
- Burkholderia cepacia
- Cystic fibrosis
- Epidemiology
- Nosocomial infections
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Microbiology
- Parasitology
- Virology
- Immunology and Allergy
- Infectious Diseases
Cite this
Burkholderia cepacia complex in cystic fibrosis and non-cystic fibrosis patients : Identification of a cluster of epidemic lineages. / Agodi, A.; Barchitta, M.; Gianninò, V.; Collura, A.; Pensabene, T.; Garlaschi, M. L.; Pasquarella, C.; Luzzaro, F.; Sinatra, F.; Mahenthiralingam, E.; Stefani, S.
In: Journal of Hospital Infection, Vol. 50, No. 3, 03.2002, p. 188-195.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Burkholderia cepacia complex in cystic fibrosis and non-cystic fibrosis patients
T2 - Identification of a cluster of epidemic lineages
AU - Agodi, A.
AU - Barchitta, M.
AU - Gianninò, V.
AU - Collura, A.
AU - Pensabene, T.
AU - Garlaschi, M. L.
AU - Pasquarella, C.
AU - Luzzaro, F.
AU - Sinatra, F.
AU - Mahenthiralingam, E.
AU - Stefani, S.
PY - 2002/3
Y1 - 2002/3
N2 - This study was performed in order to compare Burkholderia cepacia complex strains from cystic fibrosis (CF) and non-CF patients at the genomovar, genetic and epidemiological levels. A total of 92 B. cepacia respiratory tract isolates were obtained from patients attending the following CF centres: Catania and Palermo, Sicily; Gualdo Tadino, Central Italy, and Milan, Northern Italy. A total of 23 B. cepacia isolates were obtained from blood, surgical wound, and intravenous catheter sources of patients without CF, hospitalized in Catania and Varese, Northern Italy. Genomovar status identification, clonality and genetic relatedness determination, antibiotic susceptibility pattern determination and electron microscopy were performed. Transmission of infection was shown in both CF and non-CF patients by identifying clonality of responsible strains. In total 13 clones were involved in cross-transmission episodes. No outbreak was described involving both CF and non-CF patients. The present study indicates the existence of a distinct cluster of strains responsible for epidemics in CF and non-CF patients, based on their genetic relatedness, distinct from strains associated with no or negligible transmissibility. This result suggests that transmissibility is not only associated with a specific genomovar in CF patients, but also with a group of genetically related lineages in CF and non-CF patients. A key role is shown for both segregation measures and careful surveillance of infection, based on selective culture, molecular identification and epidemiological characterization of individual isolates.
AB - This study was performed in order to compare Burkholderia cepacia complex strains from cystic fibrosis (CF) and non-CF patients at the genomovar, genetic and epidemiological levels. A total of 92 B. cepacia respiratory tract isolates were obtained from patients attending the following CF centres: Catania and Palermo, Sicily; Gualdo Tadino, Central Italy, and Milan, Northern Italy. A total of 23 B. cepacia isolates were obtained from blood, surgical wound, and intravenous catheter sources of patients without CF, hospitalized in Catania and Varese, Northern Italy. Genomovar status identification, clonality and genetic relatedness determination, antibiotic susceptibility pattern determination and electron microscopy were performed. Transmission of infection was shown in both CF and non-CF patients by identifying clonality of responsible strains. In total 13 clones were involved in cross-transmission episodes. No outbreak was described involving both CF and non-CF patients. The present study indicates the existence of a distinct cluster of strains responsible for epidemics in CF and non-CF patients, based on their genetic relatedness, distinct from strains associated with no or negligible transmissibility. This result suggests that transmissibility is not only associated with a specific genomovar in CF patients, but also with a group of genetically related lineages in CF and non-CF patients. A key role is shown for both segregation measures and careful surveillance of infection, based on selective culture, molecular identification and epidemiological characterization of individual isolates.
KW - Burkholderia cepacia
KW - Cystic fibrosis
KW - Epidemiology
KW - Nosocomial infections
UR - http://www.scopus.com/inward/record.url?scp=0036512887&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036512887&partnerID=8YFLogxK
U2 - 10.1053/jhin.2001.1160
DO - 10.1053/jhin.2001.1160
M3 - Article
C2 - 11886194
AN - SCOPUS:0036512887
VL - 50
SP - 188
EP - 195
JO - Journal of Hospital Infection
JF - Journal of Hospital Infection
SN - 0195-6701
IS - 3
ER -