Abstract
Mycobacterium tuberculosis (Mtb) evades the immune response by impairing the functions of different antigen-presenting cells. We have recently shown that Mtb hijacks differentiation of monocytes into dendritic cells (DCs). To further characterize the mechanisms underlying this process, we investigated the consequences of inducing dendritic cell differentiation using interferon-α and granulocyte-macrophage colony-stimulating factor in the presence of supernatants (SNs) obtained from monocyte cultures treated with or without heat-inactivated Mtb. Although the SNs from control cultures do not interfere with the generation of fully differentiated DCs, monocytes stimulated with SNs from Mtb-stimulated cells (SN Mtb) remained CD14+ and poorly differentiated into CD1a+ cells. Among cytokines known to affect dendritic cell differentiation, we observed a robust production of interleukin-1Β, interleukin-6, interleukin-10 and tumor necrosis factor-α upon Mtb stimulation. However, only interleukin-10 neutralization through the addition of soluble interleukin-10 receptor reversed the inhibitory activity of SN Mtb. Accordingly, the addition of recombinant interleukin-10 was able to significantly reduce CD1a expression. The interaction of Mtb with differentiating monocytes rapidly activates p38 mitogen-activated protein kinase, signal transducer and activator of transcription pathways, which are likely involved in interleukin-10 gene expression. Taken together, our results suggest that Mtb may inhibit the differentiation of bystander non-infected monocytes into DCs through the release of interleukin-10. These results shed light on new aspects of the host-pathogen interaction, which might help to identify innovative immunological strategies to limit Mtb virulence.
| Original language | English |
|---|---|
| Pages (from-to) | 437-446 |
| Number of pages | 10 |
| Journal | Immunology and Cell Biology |
| Volume | 89 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 2011 |
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Keywords
- dendritic cell differentiation
- IL-10
- immunoevasion strategy
- monocyte
- Mycobacterium tuberculosis
ASJC Scopus subject areas
- Immunology
- Cell Biology
Cite this
Bystander inhibition of dendritic cell differentiation by Mycobacterium tuberculosis-induced IL-10. / Remoli, Maria Elena; Giacomini, Elena; Petruccioli, Elisa; Gafa, Valerie; Severa, Martina; Gagliardi, Maria Cristina; Iona, Elisabetta; Pine, Richard; Nisini, Roberto; Coccia, Eliana Marina.
In: Immunology and Cell Biology, Vol. 89, No. 3, 03.2011, p. 437-446.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bystander inhibition of dendritic cell differentiation by Mycobacterium tuberculosis-induced IL-10
AU - Remoli, Maria Elena
AU - Giacomini, Elena
AU - Petruccioli, Elisa
AU - Gafa, Valerie
AU - Severa, Martina
AU - Gagliardi, Maria Cristina
AU - Iona, Elisabetta
AU - Pine, Richard
AU - Nisini, Roberto
AU - Coccia, Eliana Marina
PY - 2011/3
Y1 - 2011/3
N2 - Mycobacterium tuberculosis (Mtb) evades the immune response by impairing the functions of different antigen-presenting cells. We have recently shown that Mtb hijacks differentiation of monocytes into dendritic cells (DCs). To further characterize the mechanisms underlying this process, we investigated the consequences of inducing dendritic cell differentiation using interferon-α and granulocyte-macrophage colony-stimulating factor in the presence of supernatants (SNs) obtained from monocyte cultures treated with or without heat-inactivated Mtb. Although the SNs from control cultures do not interfere with the generation of fully differentiated DCs, monocytes stimulated with SNs from Mtb-stimulated cells (SN Mtb) remained CD14+ and poorly differentiated into CD1a+ cells. Among cytokines known to affect dendritic cell differentiation, we observed a robust production of interleukin-1Β, interleukin-6, interleukin-10 and tumor necrosis factor-α upon Mtb stimulation. However, only interleukin-10 neutralization through the addition of soluble interleukin-10 receptor reversed the inhibitory activity of SN Mtb. Accordingly, the addition of recombinant interleukin-10 was able to significantly reduce CD1a expression. The interaction of Mtb with differentiating monocytes rapidly activates p38 mitogen-activated protein kinase, signal transducer and activator of transcription pathways, which are likely involved in interleukin-10 gene expression. Taken together, our results suggest that Mtb may inhibit the differentiation of bystander non-infected monocytes into DCs through the release of interleukin-10. These results shed light on new aspects of the host-pathogen interaction, which might help to identify innovative immunological strategies to limit Mtb virulence.
AB - Mycobacterium tuberculosis (Mtb) evades the immune response by impairing the functions of different antigen-presenting cells. We have recently shown that Mtb hijacks differentiation of monocytes into dendritic cells (DCs). To further characterize the mechanisms underlying this process, we investigated the consequences of inducing dendritic cell differentiation using interferon-α and granulocyte-macrophage colony-stimulating factor in the presence of supernatants (SNs) obtained from monocyte cultures treated with or without heat-inactivated Mtb. Although the SNs from control cultures do not interfere with the generation of fully differentiated DCs, monocytes stimulated with SNs from Mtb-stimulated cells (SN Mtb) remained CD14+ and poorly differentiated into CD1a+ cells. Among cytokines known to affect dendritic cell differentiation, we observed a robust production of interleukin-1Β, interleukin-6, interleukin-10 and tumor necrosis factor-α upon Mtb stimulation. However, only interleukin-10 neutralization through the addition of soluble interleukin-10 receptor reversed the inhibitory activity of SN Mtb. Accordingly, the addition of recombinant interleukin-10 was able to significantly reduce CD1a expression. The interaction of Mtb with differentiating monocytes rapidly activates p38 mitogen-activated protein kinase, signal transducer and activator of transcription pathways, which are likely involved in interleukin-10 gene expression. Taken together, our results suggest that Mtb may inhibit the differentiation of bystander non-infected monocytes into DCs through the release of interleukin-10. These results shed light on new aspects of the host-pathogen interaction, which might help to identify innovative immunological strategies to limit Mtb virulence.
KW - dendritic cell differentiation
KW - IL-10
KW - immunoevasion strategy
KW - monocyte
KW - Mycobacterium tuberculosis
UR - http://www.scopus.com/inward/record.url?scp=79952819124&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79952819124&partnerID=8YFLogxK
U2 - 10.1038/icb.2010.106
DO - 10.1038/icb.2010.106
M3 - Article
VL - 89
SP - 437
EP - 446
JO - Immunology and Cell Biology
JF - Immunology and Cell Biology
SN - 0818-9641
IS - 3
ER -