C-erbB-2 expression and DNA ploidy status in breast cancer cells obtained by fine needle aspiration (FNA)

G. Troncone, P. Zeppa, F. Fulciniti, G. Di Benedetto, M. A. Lobresco, B. De Divitiis, A. Vetrani, L. Palombini

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this study was to determine the relative rate of c-erbB-2 oncoprotein immuno-detection on matched fine needle aspiration (FNA) smears and surgical specimens of breast cancer, and to correlate the c-erbB-2 expression with the assessment of the DNA ploidy status. The expression of c-erbB-2 oncoprotein was evaluated using an immunoalkaline phosphatase technique in 49 breast aspirates (four benign and 45 malignant lesions) and 21 matched surgical specimens. The DNA ploidy status was assessed by densitometric techniques on Feulgen-stained smears. Fifty-eight per cent of the smears obtained from 45 malignant lesions and 43% of the 21 corresponding paraffin sections contained cells that were stained by the antibody. The higher incidence of c-erbB-2 expression on smears seems to be due mainly to the better antigen preservation in the fresh cytological preparations. The correlation between c-erbB-2 oncogene expression and DNA ploidy assessment showed an increased incidence of oncogene expression in aneuploid tumours (71% vs 29%; P <0.05).

Original languageEnglish
Pages (from-to)195-205
Number of pages11
JournalCytopathology
Volume4
Issue number4
Publication statusPublished - 1993

Keywords

  • Breast cancer
  • C-erbB-2 oncogene
  • DNA ploidy
  • Fine nedle aspiration

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

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