C-Fos/C-Jun expression and AP-1 activation in skin fibroblasts from centenarians

Emanuela Grassilli, Enrica Bellesia, Paolo Salomoni, Maria Antonietta Croce, Ewa Sikora, Ewa Radziszewska, Giuseppina Tesco, Marco Vergelli, Stefania Latorraca, Daniela Barbieri, Umberto Fagiolo, Silvia Santacaterina, Luigi Amaducci, Roberta Tiozzo, Sandro Sorbi, Claudio Franceschi

Research output: Contribution to journalArticlepeer-review


In vitro replicative senescence is characterized by an irreversible growth arrest due to the inability of the cell to induce some key regulators of cell cycle progression, such as c-fos and AP-1, in response to mitogenic stimuli. In vitro replicative senescence and in vivo aging have been assumed to be two related phenomena, likely controlled by overlapping or interacting genes. As a corollary, fibroblasts from centenarians, which have undergone a long process of senescence in vivo should have very limited proliferative capability. On the contrary, in a previous work we found that fibroblasts from centenarians exhibited the same capacity to respond to different mitogenic stimuli as fibroblasts from young donors. Here we provide evidences that the well preserved proliferative response is likely due to the fact that some pivotal regulators c-fos, c-jun and AP-1 - are still fully inducible, despite a long procss of in vivo senescence. Our data therefore suggest that in vivo and in vitro aging are separate phenomena whose possible relationships, if any, have to be ascertained very carefully.

Original languageEnglish
Pages (from-to)517-523
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - Sep 13 1996

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology


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