C-met activation is necessary but not sufficient for liver colonization by B16 murine melanoma cells

Shuo Lin, Dario Rusciano, Patrizia Lorenzoni, Guido Hartmann, Walter Birchmeier, Silvia Giordano, Paolo Comoglio, Max M. Burger

Research output: Contribution to journalArticlepeer-review


Metastasis to the liver is a frequent event in clinical oncology, the molecular mechanisms of which are not fully understood. We have recently reported a consistent overexpression of c-met in B16 melanoma cells selected in vivo for enhanced liver metastatic ability. In this study we address the question as to whether constitutive activation of c-met is a necessary and sufficient condition for enhanced liver colonization by B16 melanoma cells. Different levels of c-met expression and/or activation in B16 cells were achieved by subcloning, or by c-DNA transfection with either HGF/SF or the oncogenic form of c-met (tpr-met). Metastatic ability of the different populations was then evaluated in vivo by the lung colonization (experimental metastasis) assay. Results indicate that c-met (but not tpr-met) activation in B16 melanoma cells may increase their liver colonizing potential, probably by enhancing motility and invasion in response to paracrine interactions with its ligand. C-met expression per se, however, is not able to change the organ specificity of the cells. C-met activation appears instead to be required at later stages of liver colonization by B16 melanoma cells, in order to enhance their site-specific metastatic ability.

Original languageEnglish
Pages (from-to)253-265
Number of pages13
JournalClinical & Experimental Metastasis
Issue number3
Publication statusPublished - 1998


  • B16 melanoma
  • C-met
  • HGF/SF
  • Liver metastasis

ASJC Scopus subject areas

  • Cancer Research


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