C-myc and c-fos in human osteosarcoma: Prognostic value of mRNA and protein expression

Gabriella Gamberi, M. Serena Benassi, Tom Bohling, Paola Ragazzini, Lara Molendini, M. Rosa Sollazzo, Franca Pompetti, Mara Merli, Giovanna Magagnoli, Alba Balladelli, Piero Picci

Research output: Contribution to journalArticlepeer-review


The c-myc and c-fos proto-oncogenes have several putative functions, including regulation of cell growth. In many neoplasms c-myc overexpression has been linked to poor prognosis. In order to study the role of c-myc and c-fos expression on the tumorigenesis, and the metastatic spread of osteosarcoma, frozen and paraffin-embedded tissue 38 primary osteosarcoma and 10 lung metastases were analyzed. The mRNA analysis was performed by quantitative reverse transcription-polymerase chain reaction and in situ hybridization. The protein expression was studied by Western blot analysis and immunohistochemistry. C-myc and c-fos were found overexpressed in a high percentage of the relapsed tumors and of the metastases, and overexpression of both oncogenes in the same tumor was strongly correlated to the development of metastases (p <0.05), as 6 of the 7 primary tumors overexpressing both the oncogenes gave metastases. In conclusion, both c-myc and c-fos are involved in the growth and spread of osteosarcoma and a synchronous overexpression of both oncogenes is highly significant for a metastatic potential of a primary tumor.

Original languageEnglish
Pages (from-to)556-563
Number of pages8
Issue number6
Publication statusPublished - Nov 1998


  • Oncogene
  • Osteosarcoma
  • Prognosis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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