Abstract
The c-myc oncogene is implicated in normal growth and differentiation processes. Human cell lines IM9 and HepG2 stably cultured at "low" glucose concentrations (5.5 mM) show c-myc mRNA levels 3-4 times higher than cells cultured at "high" glucose concentrations (25 mM). D-fructose (a metabolizable exose) substitutes for D-glucose in reducing c-myc expression while 3-ortho-methylglucose (a non metabolizable exose) is uneffective. c-myc expression is up-regulated (by PMA) or down-regulated (by dexamethasone and long-term exposure to FCS) in human cells cultured at "low" glucose but not in cells cultured at "high" glucose. We previously demonstrated that insulin receptor gene expression in human cell lines is enhanced by glucose. Therefore, glucose controls in an opposite way the expression of two genes important in the regulation of eukaryotic cell growth and differentiation.
| Original language | English |
|---|---|
| Pages (from-to) | 1123-1129 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 165 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Dec 30 1989 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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c-myc Gene expression in human cells is controlled by glucose. / Briata, Paola; Laurino, Cinzia; Gherzi, Roberto.
In: Biochemical and Biophysical Research Communications, Vol. 165, No. 3, 30.12.1989, p. 1123-1129.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - c-myc Gene expression in human cells is controlled by glucose
AU - Briata, Paola
AU - Laurino, Cinzia
AU - Gherzi, Roberto
PY - 1989/12/30
Y1 - 1989/12/30
N2 - The c-myc oncogene is implicated in normal growth and differentiation processes. Human cell lines IM9 and HepG2 stably cultured at "low" glucose concentrations (5.5 mM) show c-myc mRNA levels 3-4 times higher than cells cultured at "high" glucose concentrations (25 mM). D-fructose (a metabolizable exose) substitutes for D-glucose in reducing c-myc expression while 3-ortho-methylglucose (a non metabolizable exose) is uneffective. c-myc expression is up-regulated (by PMA) or down-regulated (by dexamethasone and long-term exposure to FCS) in human cells cultured at "low" glucose but not in cells cultured at "high" glucose. We previously demonstrated that insulin receptor gene expression in human cell lines is enhanced by glucose. Therefore, glucose controls in an opposite way the expression of two genes important in the regulation of eukaryotic cell growth and differentiation.
AB - The c-myc oncogene is implicated in normal growth and differentiation processes. Human cell lines IM9 and HepG2 stably cultured at "low" glucose concentrations (5.5 mM) show c-myc mRNA levels 3-4 times higher than cells cultured at "high" glucose concentrations (25 mM). D-fructose (a metabolizable exose) substitutes for D-glucose in reducing c-myc expression while 3-ortho-methylglucose (a non metabolizable exose) is uneffective. c-myc expression is up-regulated (by PMA) or down-regulated (by dexamethasone and long-term exposure to FCS) in human cells cultured at "low" glucose but not in cells cultured at "high" glucose. We previously demonstrated that insulin receptor gene expression in human cell lines is enhanced by glucose. Therefore, glucose controls in an opposite way the expression of two genes important in the regulation of eukaryotic cell growth and differentiation.
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U2 - 10.1016/0006-291X(89)92719-8
DO - 10.1016/0006-291X(89)92719-8
M3 - Article
C2 - 2558650
AN - SCOPUS:0024850338
VL - 165
SP - 1123
EP - 1129
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -