Interleukin-8 (IL-8) the prototype of C-X-C chemokines stimulated the extracellular release of a phospholipase A2 (PLA2) activity in human neutrophils (PMN). The effect was concentration dependent (ECso = 12 nM) with maximal activity observed at 120 nM and rapid (60%) IL-8- and fMLP-induced release of PLA2 but did not affect the action of A23187. PLA2 activity in PMN supernatants, evaluated as release of arachidonic acid(AA) from labeled [3H]AA E. Coli membrane, was resistant to acid (pH=2) and heat (57° C for 5 min) treatments and was inhibited in the presence of 1,4dithiothreitol and scalaradial. These characteristics are consistent with the enzymatic profile of low molecular weight type II secretory PLA2. These data suggest that the release of PLA2 may contribute to the regulation of inflammatory and toxic reactions in response to C-X-C chemokines.
|Publication status||Published - 1996|
ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology