C1q as a unique player in angiogenesis with therapeutic implication in wound healing

Fleur Bossi, Claudio Tripodo, Lucia Rizzi, Roberta Bulla, Chiara Agostinis, Carla Guarnotta, Carine Munaut, Gustavo Baldassarre, Giovanni Papa, Sonia Zorzet, Berhane Ghebrehiwet, Guang Sheng Ling, Marina Botto, Francesco Tedesco

Research output: Contribution to journalArticle

Abstract

We have previously shown that C1q is expressed on endothelial cells (ECs) of newly formed decidual tissue. Here we demonstrate that C1q is deposited in wound-healing skin in the absence of C4 and C3 and that C1q mRNA is locally expressed as revealed by real-time PCR and in situ hybridization. C1q was found to induce permeability of the EC monolayer, to stimulate EC proliferation and migration, and to promote tube formation and sprouting of new vessels in a rat aortic ring assay. Using a murine model of wound healing we observed that vessel formation was defective in C1qa-/- mice and was restored to normal after local application of C1q. The mean vessel density of wound-healing tissue and the healed wound area were significantly increased in C1q-treated rats. On the basis of these results we suggest that C1q may represent a valuable therapeutic agent that can be used to treat chronic ulcers or other pathological conditions in which angiogenesis is impaired, such as myocardial ischemia.

Original languageEnglish
Pages (from-to)4209-4214
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number11
DOIs
Publication statusPublished - Mar 18 2014

Keywords

  • Animal models
  • Complement
  • Vasculogenesis

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'C1q as a unique player in angiogenesis with therapeutic implication in wound healing'. Together they form a unique fingerprint.

  • Cite this

    Bossi, F., Tripodo, C., Rizzi, L., Bulla, R., Agostinis, C., Guarnotta, C., Munaut, C., Baldassarre, G., Papa, G., Zorzet, S., Ghebrehiwet, B., Ling, G. S., Botto, M., & Tedesco, F. (2014). C1q as a unique player in angiogenesis with therapeutic implication in wound healing. Proceedings of the National Academy of Sciences of the United States of America, 111(11), 4209-4214. https://doi.org/10.1073/pnas.1311968111