TY - JOUR
T1 - C60 carboxyfullerene exerts a protective activity against oxidative stress-induced apoptosis in human peripheral blood mononuclear cells
AU - Monti, Daniela
AU - Moretti, Laura
AU - Salvioli, Stefano
AU - Straface, Elisabetta
AU - Malorni, Walter
AU - Pellicciari, Roberto
AU - Schettini, Gennaro
AU - Bisaglia, Michela
AU - Pincelli, Carlo
AU - Fumelli, Cristiana
AU - Bonafè, Massimiliano
AU - Franceschi, Claudio
PY - 2000/11/2
Y1 - 2000/11/2
N2 - C60 carboxyfullerene is a novel buckminsterfullerene-derived compound that behaves as a freeradical scavenger. In the present report, we investigated whether this drug exerts a protective activity against oxidative stress-induced apoptosis. Human peripheral blood mononuclear cells (PBMCs) were challenged by 2-deoxy-D-ribose (dRib) or TNF-α plus cycloheximide as agents that trigger apoptosis by interfering with the redox status of cell and mitochondrial membrane potential. We found that carboxyfullerene was able to protect quiescent PBMCs from apoptosis caused either by 2-deoxy-D-ribose or TNF-α plus cycloheximide by a mechanism partially involving the mitochondrial membrane potential integrity, known to be associated with early stages of apoptosis. These results represent the first indication for a target activity of buckminsterfullerenes on cells of the immune system and their mitochondria. (C) 2000 Academic Press.
AB - C60 carboxyfullerene is a novel buckminsterfullerene-derived compound that behaves as a freeradical scavenger. In the present report, we investigated whether this drug exerts a protective activity against oxidative stress-induced apoptosis. Human peripheral blood mononuclear cells (PBMCs) were challenged by 2-deoxy-D-ribose (dRib) or TNF-α plus cycloheximide as agents that trigger apoptosis by interfering with the redox status of cell and mitochondrial membrane potential. We found that carboxyfullerene was able to protect quiescent PBMCs from apoptosis caused either by 2-deoxy-D-ribose or TNF-α plus cycloheximide by a mechanism partially involving the mitochondrial membrane potential integrity, known to be associated with early stages of apoptosis. These results represent the first indication for a target activity of buckminsterfullerenes on cells of the immune system and their mitochondria. (C) 2000 Academic Press.
KW - Apoptosis
KW - Carboxyfullerenes
KW - Lymphocytes
KW - Mitochondria
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U2 - 10.1006/bbrc.2000.3715
DO - 10.1006/bbrc.2000.3715
M3 - Article
C2 - 11062018
AN - SCOPUS:0034597676
VL - 277
SP - 711
EP - 717
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -