C60 carboxyfullerene exerts a protective activity against oxidative stress-induced apoptosis in human peripheral blood mononuclear cells

Daniela Monti, Laura Moretti, Stefano Salvioli, Elisabetta Straface, Walter Malorni, Roberto Pellicciari, Gennaro Schettini, Michela Bisaglia, Carlo Pincelli, Cristiana Fumelli, Massimiliano Bonafè, Claudio Franceschi

Research output: Contribution to journalArticle

Abstract

C60 carboxyfullerene is a novel buckminsterfullerene-derived compound that behaves as a freeradical scavenger. In the present report, we investigated whether this drug exerts a protective activity against oxidative stress-induced apoptosis. Human peripheral blood mononuclear cells (PBMCs) were challenged by 2-deoxy-D-ribose (dRib) or TNF-α plus cycloheximide as agents that trigger apoptosis by interfering with the redox status of cell and mitochondrial membrane potential. We found that carboxyfullerene was able to protect quiescent PBMCs from apoptosis caused either by 2-deoxy-D-ribose or TNF-α plus cycloheximide by a mechanism partially involving the mitochondrial membrane potential integrity, known to be associated with early stages of apoptosis. These results represent the first indication for a target activity of buckminsterfullerenes on cells of the immune system and their mitochondria. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)711-717
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume277
Issue number3
DOIs
Publication statusPublished - Nov 2 2000

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Keywords

  • Apoptosis
  • Carboxyfullerenes
  • Lymphocytes
  • Mitochondria

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Monti, D., Moretti, L., Salvioli, S., Straface, E., Malorni, W., Pellicciari, R., Schettini, G., Bisaglia, M., Pincelli, C., Fumelli, C., Bonafè, M., & Franceschi, C. (2000). C60 carboxyfullerene exerts a protective activity against oxidative stress-induced apoptosis in human peripheral blood mononuclear cells. Biochemical and Biophysical Research Communications, 277(3), 711-717. https://doi.org/10.1006/bbrc.2000.3715