CA 19-9 monosialoganglioside content of human colorectal tumor cells correlates with tumor cell-induced platelet aggregation

Francesca Martini, Fiorella Guadagni, Luisa Lenti, Roberta D'Alessandro, Simona Aloe, Mario Roselli, Pier Paolo Gazzaniga, Patrizia Ferroni

Research output: Contribution to journalArticle

Abstract

Background: Gangliosides are involved in tumor cell-induced platelet aggregation (TCIPA). CA 19-9 is a monosialoganglioside detected in colon carcinoma cells. Therefore, this study was designed to evaluate whether there was any correlation between the CA 19-9 monosialoganglioside content and TCIPA. Materials and Methods: The CA 19-9 content was determined on gangliosidic extracts of human colorectal tumor cells (GEO, WiDr, DLD-1, MIP) in comparison with the degree of TCIPA. Results: CA 19-9 was detected in all cell lines, except MIP. The mean CA 19-9 content was 10.2, 30.2 and 82.6 U/μg of sialic acid for GEO, DLD-1 and WiDr, respectively. CA 19-9 content directly correlated with TCIPA (p <0.0001). Furthermore, incorporation of exogenous gangliosides in GEO cells resulted in an increase of CA 19-9 content, paralleled by a concomitant increase of TCIPA. Conclusions: The CA 19-9 monosialoganglioside may be involved in platelet/tumor cell interactions, thus playing an important role in the haematogenous metastases of colorectal cancer.

Original languageEnglish
Pages (from-to)1609-1614
Number of pages6
JournalAnticancer Research
Volume20
Issue number3 A
Publication statusPublished - 2000

Keywords

  • CA 19-9
  • Colorectal cancer
  • Gangliosides
  • Haematogenous metastases

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Fingerprint Dive into the research topics of 'CA 19-9 monosialoganglioside content of human colorectal tumor cells correlates with tumor cell-induced platelet aggregation'. Together they form a unique fingerprint.

  • Cite this