Ca2+ release induced by cyclic ADP ribose in mice lacking type 3 ryanodine receptor

Rosella Fulceri, Rosetta Rossi, Roberto Bottinelli, Antonio Conti, Elena Intravaia, Antony Galione, Angelo Benedetti, Vincenzo Sorrentino, Carlo Reggiani

Research output: Contribution to journalArticle

Abstract

The action of cyclic-ADP-ribose was studied on calcium release from sarcoplasmic reticulum of skeletal muscles of neonatal and adult wild-type and RyR3-deficient mice. cADPR increased calcium efflux from microsomes, enhanced caffeine-induced calcium release, and, in 20% of the tests, triggered calcium release in single muscle fibers. These responses occurred only in the diaphragm of adult RyR3-deficient mice. cADPR action was abolished by ryanodine, ruthenium red, and 8-brome-cADPR. These results strongly favor a specific action of cADPR on RyR1. The responsiveness of RyR1 appears in adult muscles when RyR3 is lacking.

Original languageEnglish
Pages (from-to)697-702
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume288
Issue number3
DOIs
Publication statusPublished - Nov 2 2001

Fingerprint

Cyclic ADP-Ribose
Ryanodine Receptor Calcium Release Channel
Calcium
Muscle
Bromus
Ruthenium Red
Ryanodine
Muscles
Sarcoplasmic Reticulum
Diaphragms
Microsomes
Diaphragm
Caffeine
Skeletal Muscle
Fibers

Keywords

  • CADP-ribose
  • Ryanodine receptors
  • Sarcoplasmic reticulum
  • Single muscle fibers
  • Skeletal muscles

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Ca2+ release induced by cyclic ADP ribose in mice lacking type 3 ryanodine receptor. / Fulceri, Rosella; Rossi, Rosetta; Bottinelli, Roberto; Conti, Antonio; Intravaia, Elena; Galione, Antony; Benedetti, Angelo; Sorrentino, Vincenzo; Reggiani, Carlo.

In: Biochemical and Biophysical Research Communications, Vol. 288, No. 3, 02.11.2001, p. 697-702.

Research output: Contribution to journalArticle

Fulceri, R, Rossi, R, Bottinelli, R, Conti, A, Intravaia, E, Galione, A, Benedetti, A, Sorrentino, V & Reggiani, C 2001, 'Ca2+ release induced by cyclic ADP ribose in mice lacking type 3 ryanodine receptor', Biochemical and Biophysical Research Communications, vol. 288, no. 3, pp. 697-702. https://doi.org/10.1006/bbrc.2001.5834
Fulceri, Rosella ; Rossi, Rosetta ; Bottinelli, Roberto ; Conti, Antonio ; Intravaia, Elena ; Galione, Antony ; Benedetti, Angelo ; Sorrentino, Vincenzo ; Reggiani, Carlo. / Ca2+ release induced by cyclic ADP ribose in mice lacking type 3 ryanodine receptor. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 288, No. 3. pp. 697-702.
@article{8c8a6787c3014223a03d633c472690d4,
title = "Ca2+ release induced by cyclic ADP ribose in mice lacking type 3 ryanodine receptor",
abstract = "The action of cyclic-ADP-ribose was studied on calcium release from sarcoplasmic reticulum of skeletal muscles of neonatal and adult wild-type and RyR3-deficient mice. cADPR increased calcium efflux from microsomes, enhanced caffeine-induced calcium release, and, in 20{\%} of the tests, triggered calcium release in single muscle fibers. These responses occurred only in the diaphragm of adult RyR3-deficient mice. cADPR action was abolished by ryanodine, ruthenium red, and 8-brome-cADPR. These results strongly favor a specific action of cADPR on RyR1. The responsiveness of RyR1 appears in adult muscles when RyR3 is lacking.",
keywords = "CADP-ribose, Ryanodine receptors, Sarcoplasmic reticulum, Single muscle fibers, Skeletal muscles",
author = "Rosella Fulceri and Rosetta Rossi and Roberto Bottinelli and Antonio Conti and Elena Intravaia and Antony Galione and Angelo Benedetti and Vincenzo Sorrentino and Carlo Reggiani",
year = "2001",
month = "11",
day = "2",
doi = "10.1006/bbrc.2001.5834",
language = "English",
volume = "288",
pages = "697--702",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Ca2+ release induced by cyclic ADP ribose in mice lacking type 3 ryanodine receptor

AU - Fulceri, Rosella

AU - Rossi, Rosetta

AU - Bottinelli, Roberto

AU - Conti, Antonio

AU - Intravaia, Elena

AU - Galione, Antony

AU - Benedetti, Angelo

AU - Sorrentino, Vincenzo

AU - Reggiani, Carlo

PY - 2001/11/2

Y1 - 2001/11/2

N2 - The action of cyclic-ADP-ribose was studied on calcium release from sarcoplasmic reticulum of skeletal muscles of neonatal and adult wild-type and RyR3-deficient mice. cADPR increased calcium efflux from microsomes, enhanced caffeine-induced calcium release, and, in 20% of the tests, triggered calcium release in single muscle fibers. These responses occurred only in the diaphragm of adult RyR3-deficient mice. cADPR action was abolished by ryanodine, ruthenium red, and 8-brome-cADPR. These results strongly favor a specific action of cADPR on RyR1. The responsiveness of RyR1 appears in adult muscles when RyR3 is lacking.

AB - The action of cyclic-ADP-ribose was studied on calcium release from sarcoplasmic reticulum of skeletal muscles of neonatal and adult wild-type and RyR3-deficient mice. cADPR increased calcium efflux from microsomes, enhanced caffeine-induced calcium release, and, in 20% of the tests, triggered calcium release in single muscle fibers. These responses occurred only in the diaphragm of adult RyR3-deficient mice. cADPR action was abolished by ryanodine, ruthenium red, and 8-brome-cADPR. These results strongly favor a specific action of cADPR on RyR1. The responsiveness of RyR1 appears in adult muscles when RyR3 is lacking.

KW - CADP-ribose

KW - Ryanodine receptors

KW - Sarcoplasmic reticulum

KW - Single muscle fibers

KW - Skeletal muscles

UR - http://www.scopus.com/inward/record.url?scp=0035798022&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035798022&partnerID=8YFLogxK

U2 - 10.1006/bbrc.2001.5834

DO - 10.1006/bbrc.2001.5834

M3 - Article

C2 - 11676499

AN - SCOPUS:0035798022

VL - 288

SP - 697

EP - 702

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -