Abstract
In mouse C2C12 myotubes acetylcholine (ACh) elevates the concentration of myoplasmic Ca2+ ([Ca2+]i) by inducing Ca2+ influx through transmittergated and voltage-gated channels, and by mobilizing Ca2+ from internal stores. The relative contribution of each of these ACh-activated sources to the global [Ca2+]i elevation was estimated. We found that Ca2+ entry through voltage- and ACh-gated channels accounts for roughly 80% of the total [Ca2+]i increment, while mobilization from internal caffeine-sensitive and inositol-trisphosphate (Ins P3-) sensitive stores contributes the remaining 20% to the maximal [Ca2+]i increment. Furthermore, we found that ACh-induced mobilization from Ins P3-sensitive stores also develops in embryonic chick myotubes. The differential importance of the Ca2+ signalling pathways activated by ACh during myogenesis is discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 340-345 |
| Number of pages | 6 |
| Journal | Pflugers Archiv European Journal of Physiology |
| Volume | 428 |
| Issue number | 3-4 |
| DOIs | |
| Publication status | Published - Oct 1994 |
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Keywords
- Acetylcholine
- Acetylcholine nicotinic receptor
- C2C12 myotube
- Calcium
- Metabotropic function
ASJC Scopus subject areas
- Physiology
Cite this
Ca2+ signalling pathways activated by acetylcholine in mouse C2C12 myotubes. / Grassi, Francesca; Fucile, Sergio; Eusebi, Fabrizio.
In: Pflugers Archiv European Journal of Physiology, Vol. 428, No. 3-4, 10.1994, p. 340-345.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ca2+ signalling pathways activated by acetylcholine in mouse C2C12 myotubes
AU - Grassi, Francesca
AU - Fucile, Sergio
AU - Eusebi, Fabrizio
PY - 1994/10
Y1 - 1994/10
N2 - In mouse C2C12 myotubes acetylcholine (ACh) elevates the concentration of myoplasmic Ca2+ ([Ca2+]i) by inducing Ca2+ influx through transmittergated and voltage-gated channels, and by mobilizing Ca2+ from internal stores. The relative contribution of each of these ACh-activated sources to the global [Ca2+]i elevation was estimated. We found that Ca2+ entry through voltage- and ACh-gated channels accounts for roughly 80% of the total [Ca2+]i increment, while mobilization from internal caffeine-sensitive and inositol-trisphosphate (Ins P3-) sensitive stores contributes the remaining 20% to the maximal [Ca2+]i increment. Furthermore, we found that ACh-induced mobilization from Ins P3-sensitive stores also develops in embryonic chick myotubes. The differential importance of the Ca2+ signalling pathways activated by ACh during myogenesis is discussed.
AB - In mouse C2C12 myotubes acetylcholine (ACh) elevates the concentration of myoplasmic Ca2+ ([Ca2+]i) by inducing Ca2+ influx through transmittergated and voltage-gated channels, and by mobilizing Ca2+ from internal stores. The relative contribution of each of these ACh-activated sources to the global [Ca2+]i elevation was estimated. We found that Ca2+ entry through voltage- and ACh-gated channels accounts for roughly 80% of the total [Ca2+]i increment, while mobilization from internal caffeine-sensitive and inositol-trisphosphate (Ins P3-) sensitive stores contributes the remaining 20% to the maximal [Ca2+]i increment. Furthermore, we found that ACh-induced mobilization from Ins P3-sensitive stores also develops in embryonic chick myotubes. The differential importance of the Ca2+ signalling pathways activated by ACh during myogenesis is discussed.
KW - Acetylcholine
KW - Acetylcholine nicotinic receptor
KW - C2C12 myotube
KW - Calcium
KW - Metabotropic function
UR - http://www.scopus.com/inward/record.url?scp=0028169568&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028169568&partnerID=8YFLogxK
U2 - 10.1007/BF00724516
DO - 10.1007/BF00724516
M3 - Article
C2 - 7529402
AN - SCOPUS:0028169568
VL - 428
SP - 340
EP - 345
JO - Pflugers Archiv European Journal of Physiology
JF - Pflugers Archiv European Journal of Physiology
SN - 0031-6768
IS - 3-4
ER -