Caffeine post-treatment causes a shift in the chromosome aberration types induced by mitomycin C, suggesting a caffeine-sensitive mechanism of DNA repair in G2

I. Ceccherini, N. Loprieno, I. Sbrana

Research output: Contribution to journalArticle

Abstract

Human lymphocytes were exposed in G1 to mitomycin C (2.5 μM for 2 h) and harvested at 3-h intervals from 48 to 84 h after stimulation. All cultures were also post-treated in G2 with caffeine (2mM). Different types of chromosomal aberrations were scored in the first division metaphases. Caffeine increases all chromosome aberration types by promoting a premature mitosis of damaged cells. However, when the frequency of damaged cells is not affected by the caffeine post-treatment, a reduction of the frequency of the exchange-type aberrations was shown. The possibility that caffeine interferes with some mechanism of G2 repaire is discussed.

Original languageEnglish
Pages (from-to)39-44
Number of pages6
JournalMutagenesis
Volume3
Issue number1
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Genetics
  • Health, Toxicology and Mutagenesis
  • Genetics(clinical)
  • Toxicology
  • Statistics, Probability and Uncertainty
  • Applied Mathematics

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