Human lymphocytes were exposed in G1 to mitomycin C (2.5 μM for 2 h) and harvested at 3-h intervals from 48 to 84 h after stimulation. All cultures were also post-treated in G2 with caffeine (2mM). Different types of chromosomal aberrations were scored in the first division metaphases. Caffeine increases all chromosome aberration types by promoting a premature mitosis of damaged cells. However, when the frequency of damaged cells is not affected by the caffeine post-treatment, a reduction of the frequency of the exchange-type aberrations was shown. The possibility that caffeine interferes with some mechanism of G2 repaire is discussed.
|Number of pages||6|
|Publication status||Published - 1988|
ASJC Scopus subject areas
- Health, Toxicology and Mutagenesis
- Statistics, Probability and Uncertainty
- Applied Mathematics