CALβ, a novel lipocalin associated with chondrogenesis and inflammation

Aldo Pagano, Paolo Giannoni, Adriana Zambotti, Nadia Randazzo, Barbara Zerega, Ranieri Cancedda, Beatrice Dozin

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We have previously demonstrated the association of the chicken lipocalin Ex-FABP with cartilage formation and inflammatory responses as a marker of these processes (Descalzi Cancedda et al., Biochim. Biophys. Acta 1482, 127-135, 2000). Here we report the isolation and characterisation of a new lipocalin gene laying upstream the Ex-FABP, thus representing the second member of a possible genomic cluster. This gene contains an open reading frame coding for a polypeptide of about 19 kDa. The amino-acid sequence revealed a conserved lipocalin secondary structure. Tissue distribution of the protein in developing embryos showed a preferential expression in the heart although mRNA transcripts could be detected also in muscle, lung and liver. The lowest expression was observed in the stomach, brain and skin. During endochondral formation of long bones, the protein is differentially distributed, as the transcripts, evidenced in the tibia by in situ hybridisation, are present in the hypertrophic cone of the cartilage and mostly absent in the area of the proliferating chondrocytes. Such developmental regulation was observed also in vitro in cultured chondrocytes where the transcripts were barely detectable in dedifferentiated cells but highly expressed in hypertrophic chondrocytes. The protein was also significantly induced by lipopolysaccharide stimulation of chondrocytes, indicating a possible involvement in acute phase response. Raising specific antibodies in a rabbit allowed validating, at the protein level, all the transcriptional data. Moreover, we gained evidence that the protein is actively secreted in the extracellular matrix surrounding the chondrocytes. Because of its peculiar expression in cartilage, this new protein was named chondrogenesis-associated lipocalin β (thereafter referred to as CAL β). The close similarity between Ex-FABP and CAL β expression patterns supports the hypothesis of a genomic organisation in a cluster where both genes could be co-ordinately regulated.

Original languageEnglish
Pages (from-to)264-272
Number of pages9
JournalEuropean Journal of Cell Biology
Volume81
Issue number5
Publication statusPublished - 2002

Fingerprint

Lipocalins
Chondrogenesis
Chondrocytes
Inflammation
Cartilage
Proteins
Acute-Phase Reaction
Tissue Distribution
Multigene Family
Tibia
Osteogenesis
Open Reading Frames
Genes
In Situ Hybridization
Extracellular Matrix
Lipopolysaccharides
Amino Acid Sequence
Chickens
Stomach
Embryonic Structures

Keywords

  • Chondrogenesis
  • Cluster
  • Extracellular matrix
  • Inflammation
  • Lipocalin

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Pagano, A., Giannoni, P., Zambotti, A., Randazzo, N., Zerega, B., Cancedda, R., & Dozin, B. (2002). CALβ, a novel lipocalin associated with chondrogenesis and inflammation. European Journal of Cell Biology, 81(5), 264-272.

CALβ, a novel lipocalin associated with chondrogenesis and inflammation. / Pagano, Aldo; Giannoni, Paolo; Zambotti, Adriana; Randazzo, Nadia; Zerega, Barbara; Cancedda, Ranieri; Dozin, Beatrice.

In: European Journal of Cell Biology, Vol. 81, No. 5, 2002, p. 264-272.

Research output: Contribution to journalArticle

Pagano, A, Giannoni, P, Zambotti, A, Randazzo, N, Zerega, B, Cancedda, R & Dozin, B 2002, 'CALβ, a novel lipocalin associated with chondrogenesis and inflammation', European Journal of Cell Biology, vol. 81, no. 5, pp. 264-272.
Pagano A, Giannoni P, Zambotti A, Randazzo N, Zerega B, Cancedda R et al. CALβ, a novel lipocalin associated with chondrogenesis and inflammation. European Journal of Cell Biology. 2002;81(5):264-272.
Pagano, Aldo ; Giannoni, Paolo ; Zambotti, Adriana ; Randazzo, Nadia ; Zerega, Barbara ; Cancedda, Ranieri ; Dozin, Beatrice. / CALβ, a novel lipocalin associated with chondrogenesis and inflammation. In: European Journal of Cell Biology. 2002 ; Vol. 81, No. 5. pp. 264-272.
@article{b3cfade112834164aa4a475b062bbd85,
title = "CALβ, a novel lipocalin associated with chondrogenesis and inflammation",
abstract = "We have previously demonstrated the association of the chicken lipocalin Ex-FABP with cartilage formation and inflammatory responses as a marker of these processes (Descalzi Cancedda et al., Biochim. Biophys. Acta 1482, 127-135, 2000). Here we report the isolation and characterisation of a new lipocalin gene laying upstream the Ex-FABP, thus representing the second member of a possible genomic cluster. This gene contains an open reading frame coding for a polypeptide of about 19 kDa. The amino-acid sequence revealed a conserved lipocalin secondary structure. Tissue distribution of the protein in developing embryos showed a preferential expression in the heart although mRNA transcripts could be detected also in muscle, lung and liver. The lowest expression was observed in the stomach, brain and skin. During endochondral formation of long bones, the protein is differentially distributed, as the transcripts, evidenced in the tibia by in situ hybridisation, are present in the hypertrophic cone of the cartilage and mostly absent in the area of the proliferating chondrocytes. Such developmental regulation was observed also in vitro in cultured chondrocytes where the transcripts were barely detectable in dedifferentiated cells but highly expressed in hypertrophic chondrocytes. The protein was also significantly induced by lipopolysaccharide stimulation of chondrocytes, indicating a possible involvement in acute phase response. Raising specific antibodies in a rabbit allowed validating, at the protein level, all the transcriptional data. Moreover, we gained evidence that the protein is actively secreted in the extracellular matrix surrounding the chondrocytes. Because of its peculiar expression in cartilage, this new protein was named chondrogenesis-associated lipocalin β (thereafter referred to as CAL β). The close similarity between Ex-FABP and CAL β expression patterns supports the hypothesis of a genomic organisation in a cluster where both genes could be co-ordinately regulated.",
keywords = "Chondrogenesis, Cluster, Extracellular matrix, Inflammation, Lipocalin",
author = "Aldo Pagano and Paolo Giannoni and Adriana Zambotti and Nadia Randazzo and Barbara Zerega and Ranieri Cancedda and Beatrice Dozin",
year = "2002",
language = "English",
volume = "81",
pages = "264--272",
journal = "European Journal of Cell Biology",
issn = "0171-9335",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "5",

}

TY - JOUR

T1 - CALβ, a novel lipocalin associated with chondrogenesis and inflammation

AU - Pagano, Aldo

AU - Giannoni, Paolo

AU - Zambotti, Adriana

AU - Randazzo, Nadia

AU - Zerega, Barbara

AU - Cancedda, Ranieri

AU - Dozin, Beatrice

PY - 2002

Y1 - 2002

N2 - We have previously demonstrated the association of the chicken lipocalin Ex-FABP with cartilage formation and inflammatory responses as a marker of these processes (Descalzi Cancedda et al., Biochim. Biophys. Acta 1482, 127-135, 2000). Here we report the isolation and characterisation of a new lipocalin gene laying upstream the Ex-FABP, thus representing the second member of a possible genomic cluster. This gene contains an open reading frame coding for a polypeptide of about 19 kDa. The amino-acid sequence revealed a conserved lipocalin secondary structure. Tissue distribution of the protein in developing embryos showed a preferential expression in the heart although mRNA transcripts could be detected also in muscle, lung and liver. The lowest expression was observed in the stomach, brain and skin. During endochondral formation of long bones, the protein is differentially distributed, as the transcripts, evidenced in the tibia by in situ hybridisation, are present in the hypertrophic cone of the cartilage and mostly absent in the area of the proliferating chondrocytes. Such developmental regulation was observed also in vitro in cultured chondrocytes where the transcripts were barely detectable in dedifferentiated cells but highly expressed in hypertrophic chondrocytes. The protein was also significantly induced by lipopolysaccharide stimulation of chondrocytes, indicating a possible involvement in acute phase response. Raising specific antibodies in a rabbit allowed validating, at the protein level, all the transcriptional data. Moreover, we gained evidence that the protein is actively secreted in the extracellular matrix surrounding the chondrocytes. Because of its peculiar expression in cartilage, this new protein was named chondrogenesis-associated lipocalin β (thereafter referred to as CAL β). The close similarity between Ex-FABP and CAL β expression patterns supports the hypothesis of a genomic organisation in a cluster where both genes could be co-ordinately regulated.

AB - We have previously demonstrated the association of the chicken lipocalin Ex-FABP with cartilage formation and inflammatory responses as a marker of these processes (Descalzi Cancedda et al., Biochim. Biophys. Acta 1482, 127-135, 2000). Here we report the isolation and characterisation of a new lipocalin gene laying upstream the Ex-FABP, thus representing the second member of a possible genomic cluster. This gene contains an open reading frame coding for a polypeptide of about 19 kDa. The amino-acid sequence revealed a conserved lipocalin secondary structure. Tissue distribution of the protein in developing embryos showed a preferential expression in the heart although mRNA transcripts could be detected also in muscle, lung and liver. The lowest expression was observed in the stomach, brain and skin. During endochondral formation of long bones, the protein is differentially distributed, as the transcripts, evidenced in the tibia by in situ hybridisation, are present in the hypertrophic cone of the cartilage and mostly absent in the area of the proliferating chondrocytes. Such developmental regulation was observed also in vitro in cultured chondrocytes where the transcripts were barely detectable in dedifferentiated cells but highly expressed in hypertrophic chondrocytes. The protein was also significantly induced by lipopolysaccharide stimulation of chondrocytes, indicating a possible involvement in acute phase response. Raising specific antibodies in a rabbit allowed validating, at the protein level, all the transcriptional data. Moreover, we gained evidence that the protein is actively secreted in the extracellular matrix surrounding the chondrocytes. Because of its peculiar expression in cartilage, this new protein was named chondrogenesis-associated lipocalin β (thereafter referred to as CAL β). The close similarity between Ex-FABP and CAL β expression patterns supports the hypothesis of a genomic organisation in a cluster where both genes could be co-ordinately regulated.

KW - Chondrogenesis

KW - Cluster

KW - Extracellular matrix

KW - Inflammation

KW - Lipocalin

UR - http://www.scopus.com/inward/record.url?scp=0036014854&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036014854&partnerID=8YFLogxK

M3 - Article

C2 - 12067062

AN - SCOPUS:0036014854

VL - 81

SP - 264

EP - 272

JO - European Journal of Cell Biology

JF - European Journal of Cell Biology

SN - 0171-9335

IS - 5

ER -