Calcitonin gene-related peptide (CGRP)-positive neurons and fibers in the cat periaqueductal grey matter

Fiorenzo Conti, Catia Sterninif

Research output: Contribution to journalArticle

Abstract

The morphology and topographical distribution of neurons and terminals containing calcitonin gene-related peptide (CGRP) immunoreactivity in the cat periaqueductal grey (PAG) were studied using a rabbit antiserum raised against the C-terminal region of rat αCGRP. In normal cats, numerous fibers, but rarely immunoreactive neurons, were observed in the PAG. CGRP-containing fibers showed bouton-like swellings along their length and expanded in terminal clusters of boutons. In many cases, CGRP-positive fibers were also observed in close association with small blood vessels. Immunoreactive fibers were particularly numerous at caudal PAG levels, mostly in its ventrolateral portion. In colchicine-treated cats, the pattern of CGRP-containing fibers was basically unchanged, despite a reduction of both the number of fibers and the intensity of fiber staining; in addition, numerous CGRP-positive neurons were found, mostly in the ventrolateral portion of the caudal PAG. These neurons were fusiform, spheroidal, and triangular in shape. The selective distribution of CGRP-positive elements in the PAG suggests a functional specialization of these neurons in the activation of pain-modulating mechanisms.

Original languageEnglish
Pages (from-to)497-511
Number of pages15
JournalSomatosensory & Motor Research
Volume6
Issue number5-6
DOIs
Publication statusPublished - 1989

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Keywords

  • Calcitonin gene-related peptide
  • Cat
  • Immunocytochemistry
  • Periaqueductal grey matter

ASJC Scopus subject areas

  • Physiology
  • Sensory Systems
  • Neuroscience(all)

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