Calcitonin Gene-Related Peptide (CGRP) stimulates Purkinje cell dendrite growth in culture

Simona D'Antoni, Laura Zambusi, Franca Codazzi, Daniele Zacchetti, Fabio Grohovaz, Luciano Provini, Maria Vincenza Catania, Stefano Morara

Research output: Contribution to journalArticle

Abstract

Previous reports described the transient expression during development of Calcitonin Gene-Related Peptide (CGRP) in rodent cerebellar climbing fibers and CGRP receptor in astrocytes. Here, mixed cerebellar cultures were used to analyze the effects of CGRP on Purkinje cells growth. Our results show that CGRP stimulated Purkinje cell dendrite growth under cell culture conditions mimicking Purkinje cell development in vivo. The stimulation was not blocked by CGRP8-37, a specific antagonist, suggesting the activation of other related receptors. CGRP did not affect survival of Purkinje cells, granule cells or astrocytes. The selective expression of Receptor Component Protein (RCP) (a component of CGRP receptor family) in astrocytes points to a role of these cells as mediators of CGRP effect. Finally, in pure cerebellar astrocyte cultures CGRP induced a transient morphological differentiation from flat, polygonal to stellate form. It is concluded that CGRP influences Purkinje cell dendrite growth in vitro, most likely through the involvement of astrocytes.

Original languageEnglish
Pages (from-to)2135-2143
Number of pages9
JournalNeurochemical Research
Volume35
Issue number12
DOIs
Publication statusPublished - Dec 2010

Fingerprint

Calcitonin Gene-Related Peptide
Purkinje Cells
Dendrites
Astrocytes
Growth
Calcitonin Gene-Related Peptide Receptors
Cell growth
Cell culture
Rodentia
Cell Culture Techniques
Chemical activation
Fibers
Proteins

Keywords

  • Astrocyte
  • Bergmann glia
  • CGRP receptor
  • Climbing fiber
  • Neuropeptide
  • Purkinje cells

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Biochemistry

Cite this

Calcitonin Gene-Related Peptide (CGRP) stimulates Purkinje cell dendrite growth in culture. / D'Antoni, Simona; Zambusi, Laura; Codazzi, Franca; Zacchetti, Daniele; Grohovaz, Fabio; Provini, Luciano; Catania, Maria Vincenza; Morara, Stefano.

In: Neurochemical Research, Vol. 35, No. 12, 12.2010, p. 2135-2143.

Research output: Contribution to journalArticle

D'Antoni, Simona ; Zambusi, Laura ; Codazzi, Franca ; Zacchetti, Daniele ; Grohovaz, Fabio ; Provini, Luciano ; Catania, Maria Vincenza ; Morara, Stefano. / Calcitonin Gene-Related Peptide (CGRP) stimulates Purkinje cell dendrite growth in culture. In: Neurochemical Research. 2010 ; Vol. 35, No. 12. pp. 2135-2143.
@article{a767ad33203044348bab3e05fc60c4ec,
title = "Calcitonin Gene-Related Peptide (CGRP) stimulates Purkinje cell dendrite growth in culture",
abstract = "Previous reports described the transient expression during development of Calcitonin Gene-Related Peptide (CGRP) in rodent cerebellar climbing fibers and CGRP receptor in astrocytes. Here, mixed cerebellar cultures were used to analyze the effects of CGRP on Purkinje cells growth. Our results show that CGRP stimulated Purkinje cell dendrite growth under cell culture conditions mimicking Purkinje cell development in vivo. The stimulation was not blocked by CGRP8-37, a specific antagonist, suggesting the activation of other related receptors. CGRP did not affect survival of Purkinje cells, granule cells or astrocytes. The selective expression of Receptor Component Protein (RCP) (a component of CGRP receptor family) in astrocytes points to a role of these cells as mediators of CGRP effect. Finally, in pure cerebellar astrocyte cultures CGRP induced a transient morphological differentiation from flat, polygonal to stellate form. It is concluded that CGRP influences Purkinje cell dendrite growth in vitro, most likely through the involvement of astrocytes.",
keywords = "Astrocyte, Bergmann glia, CGRP receptor, Climbing fiber, Neuropeptide, Purkinje cells",
author = "Simona D'Antoni and Laura Zambusi and Franca Codazzi and Daniele Zacchetti and Fabio Grohovaz and Luciano Provini and Catania, {Maria Vincenza} and Stefano Morara",
year = "2010",
month = "12",
doi = "10.1007/s11064-010-0294-0",
language = "English",
volume = "35",
pages = "2135--2143",
journal = "Neurochemical Research",
issn = "0364-3190",
publisher = "Springer New York",
number = "12",

}

TY - JOUR

T1 - Calcitonin Gene-Related Peptide (CGRP) stimulates Purkinje cell dendrite growth in culture

AU - D'Antoni, Simona

AU - Zambusi, Laura

AU - Codazzi, Franca

AU - Zacchetti, Daniele

AU - Grohovaz, Fabio

AU - Provini, Luciano

AU - Catania, Maria Vincenza

AU - Morara, Stefano

PY - 2010/12

Y1 - 2010/12

N2 - Previous reports described the transient expression during development of Calcitonin Gene-Related Peptide (CGRP) in rodent cerebellar climbing fibers and CGRP receptor in astrocytes. Here, mixed cerebellar cultures were used to analyze the effects of CGRP on Purkinje cells growth. Our results show that CGRP stimulated Purkinje cell dendrite growth under cell culture conditions mimicking Purkinje cell development in vivo. The stimulation was not blocked by CGRP8-37, a specific antagonist, suggesting the activation of other related receptors. CGRP did not affect survival of Purkinje cells, granule cells or astrocytes. The selective expression of Receptor Component Protein (RCP) (a component of CGRP receptor family) in astrocytes points to a role of these cells as mediators of CGRP effect. Finally, in pure cerebellar astrocyte cultures CGRP induced a transient morphological differentiation from flat, polygonal to stellate form. It is concluded that CGRP influences Purkinje cell dendrite growth in vitro, most likely through the involvement of astrocytes.

AB - Previous reports described the transient expression during development of Calcitonin Gene-Related Peptide (CGRP) in rodent cerebellar climbing fibers and CGRP receptor in astrocytes. Here, mixed cerebellar cultures were used to analyze the effects of CGRP on Purkinje cells growth. Our results show that CGRP stimulated Purkinje cell dendrite growth under cell culture conditions mimicking Purkinje cell development in vivo. The stimulation was not blocked by CGRP8-37, a specific antagonist, suggesting the activation of other related receptors. CGRP did not affect survival of Purkinje cells, granule cells or astrocytes. The selective expression of Receptor Component Protein (RCP) (a component of CGRP receptor family) in astrocytes points to a role of these cells as mediators of CGRP effect. Finally, in pure cerebellar astrocyte cultures CGRP induced a transient morphological differentiation from flat, polygonal to stellate form. It is concluded that CGRP influences Purkinje cell dendrite growth in vitro, most likely through the involvement of astrocytes.

KW - Astrocyte

KW - Bergmann glia

KW - CGRP receptor

KW - Climbing fiber

KW - Neuropeptide

KW - Purkinje cells

UR - http://www.scopus.com/inward/record.url?scp=78651304175&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78651304175&partnerID=8YFLogxK

U2 - 10.1007/s11064-010-0294-0

DO - 10.1007/s11064-010-0294-0

M3 - Article

C2 - 20960054

AN - SCOPUS:78651304175

VL - 35

SP - 2135

EP - 2143

JO - Neurochemical Research

JF - Neurochemical Research

SN - 0364-3190

IS - 12

ER -