Aumentata proliferazione dei precursori eritroidi burst forming unit-erythroid (BFU-E) indotta in vitro dal calcitriolo nell'uremia cronica: Effetto sinergico con l'eritropoietina da DNA ricombinante (rHu-Epo)

Translated title of the contribution: Calcitriol increases burst forming unit-erythroid (BFU-E) in vitro proliferation in chronic renal failure: Synergic effect with recombinant erythropoietin (rHu-Epo)

F. Aucella, G. Gatta, M. Vigilante, R. P. Scalzulli, S. Mantuano, M. Carotenuto, C. Stallone

Research output: Contribution to journalArticle

Abstract

Background. It has been suggested that calcitriol (C) could improve anemia in chronic renal failure. However it remains debatable whether vitamin D has a specific effect on erythropoiesis, or it acts via suppression of hyperparathyroidism. Methods. We enrolled 29 patients with chronic renal failure, free from malignancies, iron deficiency or other chronic or hematological diseases. Aluminium accumulation was also excluded by DFO test. 22 were on hemodialysis and 7 on conservative management, creatinine clearance ranging 22-48 ml/min. Their mean age was 62±28 years and duration of renal disease was 98±51 months. No patient underwent rHu-Epo or Vitamin D treatment. 4 subjects were enrolled as controls. Samples of peripheral blood were drawn for the Burst Forming Unit-Erythroid (BFU-E) assay. After isolation of mononuclear cells by density gradient centrifugation with Fycoll-Hypaque, a 15-day incubation was set up with four different conditions: a) adding standard dose, 3 U/ml, of r-HuEpo (Dompè Biotec), standard colture; b) combined doses of r-HuEpo, 3 U/ml, and C (Abbott), 30 pg; c) standard dose, 3 U/ml, of r-HuEpo and high dose, 300 pg, of C; and lastly d) combined high doses of r-HuEpo, 30 U/ml, and C, 300 pg. Results. In the b colture (combined low doses) a higher BFU-E proliferation was found vs standard (a) colture (33.2±15.5 vs 17.1±9.2, p

Original languageItalian
Pages (from-to)1-5
Number of pages5
JournalMinerva Urologica e Nefrologica
Volume53
Issue number1
Publication statusPublished - 2001

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ASJC Scopus subject areas

  • Nephrology
  • Urology

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