Calcium alginate particles for the combined delivery of platelet lysate and vancomycin hydrochloride in chronic skin ulcers

Michela Mori, Silvia Rossi, Maria Cristina Bonferoni, Franca Ferrari, Giuseppina Sandri, Federica Riva, Claudia Del Fante, Cesare Perotti, Carla Caramella

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of the present work was the development of a powder formulation for the combined delivery of platelet lysate and of a model antibiotic drug, vancomycin hydrochloride (VCM), in chronic skin ulcers. Calcium alginate particles were prepared by freeze-drying beads obtained by ionic gelation method. The experimental conditions adopted permitted the complete loading of VCM and of PDGF AB, the growth factor chosen as representative of those contained in PL. Such particles where able to absorb PBS (mimicking wound exudate), to form a gel and to modulate the release of VCM and of PDGF AB. They are characterized by enhancement properties of human fibroblast proliferation due to PL presence. In particular, PL, when loaded in alginate particles, was able not only to increase the number of viable cells, but also the number of cells in proliferative phase. Such properties were comparable to those of fresh PL indicating the capability of calcium alginate particles to load PL bioactive substances without altering their activity. The formulation developed is characterized by an easier and a less painful administration with respect to traditional gauzes and semisolid preparations and permits the loading in the same dosage form of active substances of different nature avoiding eventual incompatibility problems.

Original languageEnglish
Pages (from-to)505-513
Number of pages9
JournalInternational Journal of Pharmaceutics
Volume461
Issue number1-2
DOIs
Publication statusPublished - Jan 30 2014

Keywords

  • Freeze-dried beads
  • Platelet lysate
  • Skin ulcers
  • Vancomycin hydrochloride
  • Wound healing

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Medicine(all)

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