Calcium channel blockers do not prevent the anoxia/reoxygenation injury in hepatocytes

A. Gasbarrini, A. B. Borle, P. Caraceni, A. Gasbarrini, A. Facchini, M. Bernardi, D. H. Van Thiel

Research output: Contribution to journalArticle

Abstract

The effects of verapamil, nifedipine and diltiazem were studied during anoxia in freshly isolated rat hepatocytes cast in agarose gel threads and perfused with Krebs-Henseleit bicarbonate buffer (KHB). Cytosolic free calcium (Ca(i) 2+) was measured with aequorin. Anoxia was induced for 2 hr by saturating the perfusate with 95% N 2-5% CO 2. In the control group, anoxia increased Ca(i) 2+ in two distinct phases:the first rise reached a mean value of 389±35 nM (p <0.001). The second major peak reached a maximum value of 1.45 ± 0.42 μM after 1 hr (p <0.001). LDH release increased 2.5- fold during the first hr of anoxia and 6-fold during the second hr (p <0.001). During reoxygenation, Ca(i) 2+ and LDH returned to control levels within 45 min. When Ca 2+ was removed from the perfusate during the anoxic period, the second major surge in Ca(i) 2+ was totally abolished and the rise in LDH release was significantly reduced Ca 2+ channel blockers did not prevent the increase in Ca(i) 2+ and the rise in LDH release. On the contrary, high concentrations of nifedipine and diltiazem significantly increased anoxic cell injury as measured by LDH and trypan blue uptake. Since the rise in Ca(i) 2+ was not suppressed by the 3 Ca 2+ antagonists used in these studies, Ca 2+ channels of the L-type are probably not involved in the massive increase in Ca(i) 2+ evoked by anoxia. These studies also suggest that the cell injury and LDH release induced by anoxia may be related to the increase in Ca(i) 2+.

Original languageEnglish
Pages (from-to)127-131
Number of pages5
JournalTransplantationsmedizin: Organ der Deutschen Transplantationsgesellschaft
Volume6
Issue number2
Publication statusPublished - 1994

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Calcium Channel Blockers
Hepatocytes
Wounds and Injuries
Diltiazem
Nifedipine
Aequorin
Trypan Blue
Carbon Monoxide
Bicarbonates
Verapamil
Sepharose
Hypoxia
Gels
Calcium
Control Groups

ASJC Scopus subject areas

  • Immunology and Allergy
  • Transplantation

Cite this

Gasbarrini, A., Borle, A. B., Caraceni, P., Gasbarrini, A., Facchini, A., Bernardi, M., & Van Thiel, D. H. (1994). Calcium channel blockers do not prevent the anoxia/reoxygenation injury in hepatocytes. Transplantationsmedizin: Organ der Deutschen Transplantationsgesellschaft, 6(2), 127-131.

Calcium channel blockers do not prevent the anoxia/reoxygenation injury in hepatocytes. / Gasbarrini, A.; Borle, A. B.; Caraceni, P.; Gasbarrini, A.; Facchini, A.; Bernardi, M.; Van Thiel, D. H.

In: Transplantationsmedizin: Organ der Deutschen Transplantationsgesellschaft, Vol. 6, No. 2, 1994, p. 127-131.

Research output: Contribution to journalArticle

Gasbarrini, A, Borle, AB, Caraceni, P, Gasbarrini, A, Facchini, A, Bernardi, M & Van Thiel, DH 1994, 'Calcium channel blockers do not prevent the anoxia/reoxygenation injury in hepatocytes', Transplantationsmedizin: Organ der Deutschen Transplantationsgesellschaft, vol. 6, no. 2, pp. 127-131.
Gasbarrini, A. ; Borle, A. B. ; Caraceni, P. ; Gasbarrini, A. ; Facchini, A. ; Bernardi, M. ; Van Thiel, D. H. / Calcium channel blockers do not prevent the anoxia/reoxygenation injury in hepatocytes. In: Transplantationsmedizin: Organ der Deutschen Transplantationsgesellschaft. 1994 ; Vol. 6, No. 2. pp. 127-131.
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AU - Van Thiel, D. H.

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