Calcium-dependent DNA fragmentation in human synovial cells exposed to cold shock

Mariarosa Perotti, Filippo Toddei, Francesca Mirabelli, Mariapia Vairetti, Giorgo Bellomo, David J. McConkey, Sten Orrenius

Research output: Contribution to journalArticle

Abstract

Exposure of confluent human synovial McCoy's cells to near-freezing temperatures followed by rewarming at 37°C resulted in endonuclease activation and cell death characteristic of a suicide process known as apoptosis. Both DNA fragmentation and cell killing were dependent on a sustained increase in the cytosolic Ca2+ concentration. Sensitivity to cold shock-induced endonuclease activation was critically dependent on the cell cycle (proliferative) status and limited to confluent cells, whereas cells in the logarithmic growth phase were completely resistant. However, DNA fragmentation was promoted in the proliferating McCoy's cells pretreated with H-7 or sphingosine, inhibitors of protein kinase C. In addition, phorbol ester, known to activate PKC, inhibited DNA fragmentation in the confluent cells. Our findings indicate that cold shock-induced DNA fragmentation in McCoy's cells is dependent on a sustained Ca2+ increase, and sensitivity to the process appears to be regulated by the status of protein kinase C.

Original languageEnglish
Pages (from-to)331-334
Number of pages4
JournalFEBS Letters
Volume259
Issue number2
DOIs
Publication statusPublished - Jan 1 1990

Fingerprint

DNA Fragmentation
Shock
Calcium
Endonucleases
DNA
Protein Kinase C
Chemical activation
Cells
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Sphingosine
Phorbol Esters
Cell death
Freezing
Rewarming
Apoptosis
Suicide
Cell Cycle
Cell Death
Temperature
Growth

Keywords

  • Apoptosis
  • Calmodulin
  • Endonuclease
  • Protein kinase C

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Perotti, M., Toddei, F., Mirabelli, F., Vairetti, M., Bellomo, G., McConkey, D. J., & Orrenius, S. (1990). Calcium-dependent DNA fragmentation in human synovial cells exposed to cold shock. FEBS Letters, 259(2), 331-334. https://doi.org/10.1016/0014-5793(90)80040-P

Calcium-dependent DNA fragmentation in human synovial cells exposed to cold shock. / Perotti, Mariarosa; Toddei, Filippo; Mirabelli, Francesca; Vairetti, Mariapia; Bellomo, Giorgo; McConkey, David J.; Orrenius, Sten.

In: FEBS Letters, Vol. 259, No. 2, 01.01.1990, p. 331-334.

Research output: Contribution to journalArticle

Perotti, M, Toddei, F, Mirabelli, F, Vairetti, M, Bellomo, G, McConkey, DJ & Orrenius, S 1990, 'Calcium-dependent DNA fragmentation in human synovial cells exposed to cold shock', FEBS Letters, vol. 259, no. 2, pp. 331-334. https://doi.org/10.1016/0014-5793(90)80040-P
Perotti M, Toddei F, Mirabelli F, Vairetti M, Bellomo G, McConkey DJ et al. Calcium-dependent DNA fragmentation in human synovial cells exposed to cold shock. FEBS Letters. 1990 Jan 1;259(2):331-334. https://doi.org/10.1016/0014-5793(90)80040-P
Perotti, Mariarosa ; Toddei, Filippo ; Mirabelli, Francesca ; Vairetti, Mariapia ; Bellomo, Giorgo ; McConkey, David J. ; Orrenius, Sten. / Calcium-dependent DNA fragmentation in human synovial cells exposed to cold shock. In: FEBS Letters. 1990 ; Vol. 259, No. 2. pp. 331-334.
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