Calcium-dependent glutamate release during neuronal development and synaptogenesis: Different involvement of ω-agatoxin IVA- and ω-conotoxin GVIA-sensitive channels

Claudia Verderio, Silvia Coco, Guido Fumagalli, Michela Matteoli

Research output: Contribution to journalArticlepeer-review

Abstract

Hippocampal neurons maintained in primary culture recycle synaptic vesicles and express functional glutamate receptors since early stages of neuronal development. By analyzing glutamate-induced cytosolic calcium changes to sense presynaptically released neurotransmitter, we demonstrate that the ability of neurons to release glutamate in the extracellular space is temporally coincident with the property of synaptic vesicles to undergo exocytotic-endocytotic recycling. Neuronal differentiation and maturation of synaptic contacts coincide with a change in the subtype of calcium channels primarily involved in controlling neurosecretion. Whereas ω-agatoxin IVA- sensitive channels play a role in controlling neurotransmitter secretion at all stages of neuronal differentiation, ω-conotoxin GVIA-sensitive channels are primarily involved in mediating glutamate release at early developmental stages only.

Original languageEnglish
Pages (from-to)6449-6453
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number14
DOIs
Publication statusPublished - Jul 3 1995

ASJC Scopus subject areas

  • General
  • Genetics

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