TY - JOUR
T1 - Calcium-dependent glutamate release during neuronal development and synaptogenesis
T2 - Different involvement of ω-agatoxin IVA- and ω-conotoxin GVIA-sensitive channels
AU - Verderio, Claudia
AU - Coco, Silvia
AU - Fumagalli, Guido
AU - Matteoli, Michela
PY - 1995/7/3
Y1 - 1995/7/3
N2 - Hippocampal neurons maintained in primary culture recycle synaptic vesicles and express functional glutamate receptors since early stages of neuronal development. By analyzing glutamate-induced cytosolic calcium changes to sense presynaptically released neurotransmitter, we demonstrate that the ability of neurons to release glutamate in the extracellular space is temporally coincident with the property of synaptic vesicles to undergo exocytotic-endocytotic recycling. Neuronal differentiation and maturation of synaptic contacts coincide with a change in the subtype of calcium channels primarily involved in controlling neurosecretion. Whereas ω-agatoxin IVA- sensitive channels play a role in controlling neurotransmitter secretion at all stages of neuronal differentiation, ω-conotoxin GVIA-sensitive channels are primarily involved in mediating glutamate release at early developmental stages only.
AB - Hippocampal neurons maintained in primary culture recycle synaptic vesicles and express functional glutamate receptors since early stages of neuronal development. By analyzing glutamate-induced cytosolic calcium changes to sense presynaptically released neurotransmitter, we demonstrate that the ability of neurons to release glutamate in the extracellular space is temporally coincident with the property of synaptic vesicles to undergo exocytotic-endocytotic recycling. Neuronal differentiation and maturation of synaptic contacts coincide with a change in the subtype of calcium channels primarily involved in controlling neurosecretion. Whereas ω-agatoxin IVA- sensitive channels play a role in controlling neurotransmitter secretion at all stages of neuronal differentiation, ω-conotoxin GVIA-sensitive channels are primarily involved in mediating glutamate release at early developmental stages only.
UR - http://www.scopus.com/inward/record.url?scp=0029023902&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029023902&partnerID=8YFLogxK
U2 - 10.1073/pnas.92.14.6449
DO - 10.1073/pnas.92.14.6449
M3 - Article
C2 - 7604011
AN - SCOPUS:0029023902
VL - 92
SP - 6449
EP - 6453
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 14
ER -