Calcium-dependent transmitter secretion reconstituted in Xenopus oocytes: Requirement for synaptophysin

Janet Alder, Bai Lu, Flavia Valtorta, Paul Greengard, Mu Ming Poo

Research output: Contribution to journalArticlepeer-review

Abstract

Calcium-dependent glutamate secretion was reconstituted in Xenopus oocytes by injecting the oocyte with total rat cerebellar messenger RNA (mRNA). Co-injection of total mRNA with antisense oligonucleotides to synaptophysin message decreased the expression of synaptophysin in the oocyte and reduced the calcium- dependent secretion. A similar effect on secretion was observed for oocytes injected with total mRNA together with an antibody to rat synaptophysin. These results indicate that synaptophysin is necessary for transmitter secretion and that the oocyte expression system may be useful for dissecting the molecular events associated with the secretory process.

Original languageEnglish
Pages (from-to)657-661
Number of pages5
JournalScience
Volume257
Issue number5070
Publication statusPublished - 1992

ASJC Scopus subject areas

  • General

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