Calcium responses in human fibroblasts: A diagnostic molecular profile for Alzheimer's disease

Naohide Hirashima, René Etcheberrigaray, Stefania Bergamaschi, Marco Racchi, Fiorenzo Battaini, Giuliano Binetti, Stefano Govoni, Daniel L. Alkon

Research output: Contribution to journalArticlepeer-review

Abstract

We have previously identified alterations of K+ channel function, IP3- mediated calcium release, and Cp20 (a memory associated GTP binding protein) in fibroblasts from AD patients vs. controls. In the present study we introduce a scoring system based on these response alterations that integrates two or more alterations (and their degree) in AD vs. control fibroblasts. This scoring system generates an index that distinguishes AD patients from controls with both high specificity and sensitivity. We also show that low doses of bradykinin elicit intracellular calcium release almost exclusively in AD cell lines in an all or none fashion that provide a clear measurement of enhanced IP3-mediated function in AD vs. controls.

Original languageEnglish
Pages (from-to)549-555
Number of pages7
JournalNeurobiology of Aging
Volume17
Issue number4
DOIs
Publication statusPublished - 1996

Keywords

  • Alzheimer's disease
  • Bombesin
  • Bradykinin
  • Calcium
  • Fibroblast
  • TEA

ASJC Scopus subject areas

  • Clinical Neurology
  • Biological Psychiatry
  • Developmental Neuroscience
  • Neurology
  • Psychology(all)

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