Abstract
Immunohistochemistry is a powerful diagnostic adjunct in the differential diagnosis between malignant mesothelioma (especially of the epithelial type) and adenocarcinoma metastatic to the serous membranes. Most of the immunological probes commonly used, however, recognize antigens expressed by the epithelial malignancies and absent from mesothelial cells and mesotheliomas. Probes suitable for the positive identification of mesotheliomas are comparatively scarce and much less commonly used because of their reduced sensitivity and specificity, their unsuitability for staining routinely fixed and embedded tissues, or their lack of commercial availability. We now document that two different polyclonal antisera to calretinin consistently immunostain mesothelial cells and malignant mesotheliomas both in routinely fixed and embedded tissue sections and in cytological preparations of serous effusions. The diagnostic sensitivity of this novel immunocytochemical approach reached 100%, allowing immunostaining of all 44 mesotheliomas investigated, which included five biphasic and three sarcomatoid types. The specificity of calretinin immunoreactivity was checked against 294 adenocarcinomas of different origin (19 serosal metastases and 275 primary tumors potentially able to metastatize to serosal membranes) relevant for the discussion of the differential diagnosis with malignant mesothelioma: only 28 cases showed focal immunoreactivity for calretinin. We conclude that calretinin is a most useful marker for the positive identification of malignant mesotheliomas.
| Original language | English |
|---|---|
| Pages (from-to) | 1037-1046 |
| Number of pages | 10 |
| Journal | American Journal of Surgical Pathology |
| Volume | 20 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Sep 1996 |
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Keywords
- Calretinin
- Immunocytochemical markers for mesothelioma
- Immunohistochemistry
- Malignant mesothelioma
- Metastatic adenocarcinoma
ASJC Scopus subject areas
- Anatomy
- Pathology and Forensic Medicine
Cite this
Calretinin : A novel immunocytochemical marker for mesothelioma. / Doglioni, Claudio; Dei Tos, Angelo P.; Laurino, Licia; Luzzolino, Paolo; Chiarelli, Concetta; Celio, Marco R.; Viale, Giuseppe; RcPath, M.
In: American Journal of Surgical Pathology, Vol. 20, No. 9, 09.1996, p. 1037-1046.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Calretinin
T2 - A novel immunocytochemical marker for mesothelioma
AU - Doglioni, Claudio
AU - Dei Tos, Angelo P.
AU - Laurino, Licia
AU - Luzzolino, Paolo
AU - Chiarelli, Concetta
AU - Celio, Marco R.
AU - Viale, Giuseppe
AU - RcPath, M.
PY - 1996/9
Y1 - 1996/9
N2 - Immunohistochemistry is a powerful diagnostic adjunct in the differential diagnosis between malignant mesothelioma (especially of the epithelial type) and adenocarcinoma metastatic to the serous membranes. Most of the immunological probes commonly used, however, recognize antigens expressed by the epithelial malignancies and absent from mesothelial cells and mesotheliomas. Probes suitable for the positive identification of mesotheliomas are comparatively scarce and much less commonly used because of their reduced sensitivity and specificity, their unsuitability for staining routinely fixed and embedded tissues, or their lack of commercial availability. We now document that two different polyclonal antisera to calretinin consistently immunostain mesothelial cells and malignant mesotheliomas both in routinely fixed and embedded tissue sections and in cytological preparations of serous effusions. The diagnostic sensitivity of this novel immunocytochemical approach reached 100%, allowing immunostaining of all 44 mesotheliomas investigated, which included five biphasic and three sarcomatoid types. The specificity of calretinin immunoreactivity was checked against 294 adenocarcinomas of different origin (19 serosal metastases and 275 primary tumors potentially able to metastatize to serosal membranes) relevant for the discussion of the differential diagnosis with malignant mesothelioma: only 28 cases showed focal immunoreactivity for calretinin. We conclude that calretinin is a most useful marker for the positive identification of malignant mesotheliomas.
AB - Immunohistochemistry is a powerful diagnostic adjunct in the differential diagnosis between malignant mesothelioma (especially of the epithelial type) and adenocarcinoma metastatic to the serous membranes. Most of the immunological probes commonly used, however, recognize antigens expressed by the epithelial malignancies and absent from mesothelial cells and mesotheliomas. Probes suitable for the positive identification of mesotheliomas are comparatively scarce and much less commonly used because of their reduced sensitivity and specificity, their unsuitability for staining routinely fixed and embedded tissues, or their lack of commercial availability. We now document that two different polyclonal antisera to calretinin consistently immunostain mesothelial cells and malignant mesotheliomas both in routinely fixed and embedded tissue sections and in cytological preparations of serous effusions. The diagnostic sensitivity of this novel immunocytochemical approach reached 100%, allowing immunostaining of all 44 mesotheliomas investigated, which included five biphasic and three sarcomatoid types. The specificity of calretinin immunoreactivity was checked against 294 adenocarcinomas of different origin (19 serosal metastases and 275 primary tumors potentially able to metastatize to serosal membranes) relevant for the discussion of the differential diagnosis with malignant mesothelioma: only 28 cases showed focal immunoreactivity for calretinin. We conclude that calretinin is a most useful marker for the positive identification of malignant mesotheliomas.
KW - Calretinin
KW - Immunocytochemical markers for mesothelioma
KW - Immunohistochemistry
KW - Malignant mesothelioma
KW - Metastatic adenocarcinoma
UR - http://www.scopus.com/inward/record.url?scp=0029819215&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029819215&partnerID=8YFLogxK
U2 - 10.1097/00000478-199609000-00001
DO - 10.1097/00000478-199609000-00001
M3 - Article
VL - 20
SP - 1037
EP - 1046
JO - American Journal of Surgical Pathology
JF - American Journal of Surgical Pathology
SN - 0147-5185
IS - 9
ER -