cAMP-dependent induction of PDE5 expression in murine neuroblastoma cell differentiation

D. Giordano, M. Giorgi, C. Sette, S. Biagioni, G. Augusti-Tocco

Research output: Contribution to journalArticlepeer-review


The present study demonstrates, in both hybrid NG108-15 and mouse neuroblastoma N18TG2 cells, the presence and regulation of PDE5 mRNA during cell differentiation. PDE5 cDNA probes in Northern blot analysis recognize a ~9 kb transcript in bovine lung as well as in mouse neuroblastoma cells. Hybridization on total RNA extracted from dibutyryl-cAMP-treated NG108-15 cells shows a 5-fold increase of PDE5 9 kb mRNA: such an increase is not observed in N18TG2 although we observed a similar increase in the enzymatic activity of both cell lines. Our data demonstrate that PDE5 gene expression can be regulated by cAMP and suggest the existence of a complex regulatory system for PDE5 activity. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)218-222
Number of pages5
JournalFEBS Letters
Issue number2-3
Publication statusPublished - Mar 12 1999


  • cAMP-dependent expression
  • Differentiation
  • Neuroblastoma
  • NG108-15
  • PDE5

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology


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