Cancer gastric chemoprevention: Isolation of gastric tumor-initiating cells

Federica Mori, Valeria Canu, Laura Lorenzon, Alfredo Garofalo, Giovanni Blandino, Sabrina Strano

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Gastric cancer is an important healthcare problem and represents the second leading cause of death for malignant disease worldwide. In the Western world, the diagnosis is done at late stage when treatments can be only palliative. Searches for new therapeutic regimens as well as for new biomarkers are in progress. To reduce cancer mortality is crucial the prevention of the lesion at earlier stages. Therefore, new bullets to prevention are needed. Nowadays, studies relating to different kinds of tumor are unanimous in considering cancer stem cells (CSCs) as "the core" of the tumor and the responsible of tumor chemoresistance and relapse. This chapter aims to provide the instructions to (1) isolate, (2) grow, and (3) validate, both in vivo and in vitro, the gastric CSC subpopulation.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages129-137
Number of pages9
Volume1379
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1379
ISSN (Print)10643745

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Keywords

  • Cancer stem cells
  • Chemoprevention
  • Chemoresistance
  • Gastric cancer
  • Patient-derived xenograft

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Mori, F., Canu, V., Lorenzon, L., Garofalo, A., Blandino, G., & Strano, S. (2016). Cancer gastric chemoprevention: Isolation of gastric tumor-initiating cells. In Methods in Molecular Biology (Vol. 1379, pp. 129-137). (Methods in Molecular Biology; Vol. 1379). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3191-0_12