Candida parapsilosis bloodstream infection in pediatric oncology patients

Results of an epidemiologic investigation

Brunella Posteraro, Stefania Bruno, Stefania Boccia, Antonio Ruggiero, Maurizio Sanguinetti, Vincenzo Romano Spica, Gualtiero Ricciardi, Giovanni Fadda

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

OBJECTIVE: To investigate an outbreak of Candida parapsilosis bloodstream infections (BSIs) involving three patients admitted to a pediatric oncology unit between April and June 2002. METHODS: After the third case was documented, cultures were performed of the hands of all medical and paramedical staff members in the pediatric oncology unit and of environmental surfaces in the rooms occupied by the three patients. Electrophoretic karyotyping with pulsed-field gel electrophoresis and arbitrarily primed polymerase chain reaction were used to assess the genetic relatedness among C. parapsilosis isolates. RESULTS: The three cases of C. parapsilosis BSI were diagnosed based on blood cultures performed during a 38-day period. Evidence of prior C. parapsilosis colonization of the gastrointestinal tract was present in only the first case. Each patient had an indwelling central venous catheter (CVC), which was promptly removed, and semiquantitative catheter tip cultures also revealed C. parapsilosis. None of the 30 environmental cultures performed was positive for C. parapsilosis, but the fungus was isolated from the hands of 6 of the 20 nurses tested. Both molecular typing methods revealed identical DNA fingerprinting patterns for all 13 patient isolates (7 from blood, 3 from CVC tips, and 3 from the gastrointestinal tract) and for 5 of the 6 recovered from the nurses' hands. CONCLUSIONS: These findings suggest the possibility of cross-infection with a single C. parapsilosis strain that was transmitted (probably during CVC dressing changes) by nurses whose hands were colonized with it. The role of previous gastrointestinal colonization in the first case cannot be excluded.

Original languageEnglish
Pages (from-to)641-645
Number of pages5
JournalInfection Control and Hospital Epidemiology
Volume25
Issue number8
DOIs
Publication statusPublished - Aug 2004

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Candida
Central Venous Catheters
Hand
Pediatrics
Nurses
Infection
Gastrointestinal Tract
Molecular Typing
Karyotyping
Indwelling Catheters
DNA Fingerprinting
Pulsed Field Gel Electrophoresis
Medical Staff
Bandages
Cross Infection
Disease Outbreaks
Fungi
Catheters
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology

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Candida parapsilosis bloodstream infection in pediatric oncology patients : Results of an epidemiologic investigation. / Posteraro, Brunella; Bruno, Stefania; Boccia, Stefania; Ruggiero, Antonio; Sanguinetti, Maurizio; Spica, Vincenzo Romano; Ricciardi, Gualtiero; Fadda, Giovanni.

In: Infection Control and Hospital Epidemiology, Vol. 25, No. 8, 08.2004, p. 641-645.

Research output: Contribution to journalArticle

Posteraro, Brunella ; Bruno, Stefania ; Boccia, Stefania ; Ruggiero, Antonio ; Sanguinetti, Maurizio ; Spica, Vincenzo Romano ; Ricciardi, Gualtiero ; Fadda, Giovanni. / Candida parapsilosis bloodstream infection in pediatric oncology patients : Results of an epidemiologic investigation. In: Infection Control and Hospital Epidemiology. 2004 ; Vol. 25, No. 8. pp. 641-645.
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